Authors :
Sudharsan Varatharajan; Sudhanva Devaprasad Dixit
Volume/Issue :
Volume 8 - 2023, Issue 8 - August
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/59v5yu7b
DOI :
https://doi.org/10.5281/zenodo.8252330
Abstract :
The zebrafish (Danio rerio) has emerged as an
invaluable model organism for studying vertebrate
development due to its transparency, rapid development,
and genetic tractability. Sodium chloride (NaCl) is an
essential component of the aquatic environment and
plays a critical role in maintaining osmotic balance in
fish. However, excessive salinity levels in aquatic
ecosystems due to anthropogenic activities have raised
concerns about their impact on aquatic organisms,
particularly during early life stages. This experimental
research was performed to investigate the effect of
sodium chloride (common salt) on the embryonic
development of Zebrafish (Danio rerio), and to examine
the embryos before and after the treatment with Sodium
Chloride (NaCl), and analyze the embryos using ImageJ
tool. Zebrafishes were used in this experiment as they
are considered as powerful model organisms, which
means that the information obtained through them can
help us better understand how Sodium affects the human
embryos. Zebrafish embryos were placed in a tray, then
the embryos were subjected to saline solutions at various
concentrations for 24 hours during the experiment,
including 0.002 g/mL, and 0.02 g/mL of NaCl.
Furthermore, the dead embryos were separated out, data
and observations were recorded. ImageJ software was
used to analyze the early-hatched zebrafish embryo. The
result of this experiment demonstrated that 0.002 g/ml of
Sodium chloride is the ideal salt concentration for early
hatching of zebrafish embryos and it has no side effects
on Zebrafish embryos. The findings of this experiment
suggested that Sodium chloride has minute or no side
effects on the growth of human embryos, but further
investigation needs to be performed.
Keywords :
Zebrafish; sodium Chloride; Embryonic Development; Minimal Dose; Lethal Dose; imageJ Software.
The zebrafish (Danio rerio) has emerged as an
invaluable model organism for studying vertebrate
development due to its transparency, rapid development,
and genetic tractability. Sodium chloride (NaCl) is an
essential component of the aquatic environment and
plays a critical role in maintaining osmotic balance in
fish. However, excessive salinity levels in aquatic
ecosystems due to anthropogenic activities have raised
concerns about their impact on aquatic organisms,
particularly during early life stages. This experimental
research was performed to investigate the effect of
sodium chloride (common salt) on the embryonic
development of Zebrafish (Danio rerio), and to examine
the embryos before and after the treatment with Sodium
Chloride (NaCl), and analyze the embryos using ImageJ
tool. Zebrafishes were used in this experiment as they
are considered as powerful model organisms, which
means that the information obtained through them can
help us better understand how Sodium affects the human
embryos. Zebrafish embryos were placed in a tray, then
the embryos were subjected to saline solutions at various
concentrations for 24 hours during the experiment,
including 0.002 g/mL, and 0.02 g/mL of NaCl.
Furthermore, the dead embryos were separated out, data
and observations were recorded. ImageJ software was
used to analyze the early-hatched zebrafish embryo. The
result of this experiment demonstrated that 0.002 g/ml of
Sodium chloride is the ideal salt concentration for early
hatching of zebrafish embryos and it has no side effects
on Zebrafish embryos. The findings of this experiment
suggested that Sodium chloride has minute or no side
effects on the growth of human embryos, but further
investigation needs to be performed.
Keywords :
Zebrafish; sodium Chloride; Embryonic Development; Minimal Dose; Lethal Dose; imageJ Software.
