Endodontic Biomarkers: A Paradigm Shift in Endodontic Diagnosis


Authors : Dr. K Shreya Shetty; Dr. Swathi Amin

Volume/Issue : Volume 10 - 2025, Issue 7 - July


Google Scholar : https://tinyurl.com/23tpupvk

Scribd : https://tinyurl.com/222ddk7x

DOI : https://doi.org/10.38124/ijisrt/25jul1894

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Abstract : For evaluating the accuracy of vital pulp therapy for an inflamed pulp, the use of non-invasive diagnostic tools are essential to avoid further damage to the pulp dentin complex. The identification of specific biomarkers reflecting the inflammatory status of the dental pulp holds significant promise in guiding accurate diagnosis and enabling personalized treatment strategies. This review article focuses upon delivering brief information on endodontic biomarkers seen in different pulpal and peri apical pathologies and their isolation techniques.

Keywords : Endodontic Biomarkers, Periapical Inflammation, Apical Periodontitis, Diagnostic Test.

References :

  1. Rechenberg D-K, Galicia JC, Peters OA (2016) Biological Markers for Pulpal Inflammation: A Systematic Review. PLoS ONE 11(11): e0167289. doi: 10.1371/journal.pone.0167289.
  2. Hahn CL, Liewehr FR. Relationships between caries bacteria, host responses, and clinical signs and symptoms of pulpitis. J Endod 2007; 33:213‑39.
  3. Ricucci D, Loghin S, Siqueira JF Jr. Correlation between clinical and histologic pulp diagnoses. J Endod 2014; 40:1932‑29.
  4. Zanini M, Meyer E, Simon S. Pulp inflammation diagnosis from clinical to inflammatory mediators: A systematic review. J Endod 2017; 43:1033‑51.
  5. Biomarkers Definitions Working Group, Atkinson Jr AJ, Colburn WA, DeGruttola VG, DeMets DL, Downing GJ, Hoth DF, Oates JA, Peck CC, Schooley RT, Spilker BA. Biomarkers and surrogate endpoints: preferred definitions and conceptual framework. Clinical pharmacology & therapeutics. 2001 Mar;69(3):89-95.
  6. Mente J, Petrovic J, Gehrig H, Rampf S, Michel A, Schürz A, et al. A prospective clinical pilot study on the level of matrix metalloproteinase‑9 in dental pulpal blood as a marker for the state of inflammation in the pulp tissue. J Endod 2016; 42:190‑97.
  7. Rechenberg DK, Bostanci N, Zehnder M, Belibasakis GN. Periapical fluid RANKL and IL‑8 are differentially regulated in pulpitis and apical periodontitis. Cytokine 2014; 69:116‑69.
  8. Zehnder M, Rechenberg DK, Bostanci N, Sisman F, Attin T. Comparison of vehicles to collect dentinal fluid for molecular analysis. J Dent 2014; 42:1027‑32.
  9. Gomes MS, Blattner TC, Sant’Ana Filho M, Grecca FS, Hugo FN, Fouad AF, et al. Can apical periodontitis modify systemic levels of inflammatory markers? A systematic review and meta‑analysis. J Endod 2013; 39:1205‑17.
  10. Garrido M, Dezerega A, Bordagaray MJ, Reyes M, Vernal R, Melgar‑Rodríguez S, et al. C‑reactive protein expression is up‑regulated in apical lesions of endodontic origin in association with interleukin‑6. J Endod 2015; 41:464‑49.
  11. Zehnder M, Wegehaupt FJ, Attin T. A first study on the usefulness of matrix metalloproteinase 9 from dentinal fluid to indicate pulp inflammation. J Endod 2011; 37:17‑20.
  12. Abd‑Elmeguid A, Abdeldayem M, Kline LW, Moqbel R, Vliagoftis H, Yu DC. Osteocalcin expression in pulp inflammation. J Endod 2013; 39:865‑72.
  13. Satnam S. Virdee, Nasir Z. Bashir, Milan Krstic Josette Camilleri, Melissa M.  Grant Paul R. Coper Philip L. Tomson. Periarticular tissue fluid derived biomarkers for apical periodontitis: An invitro methodological and In vivo Cross -sectional Study. Int Endod J 2023;56 (10): 1222-40.
  14. Maita E, Simpson MD, Tao L, Pashley DH (1991) Fluid and protein flux across the pulp dentine complex of the dog in vivo. Archives of Oral Biology 36, 103–110.
  15. Bostanci N, Heywood W, Mills K, Parkar M, Nibali L, Donos N. Application of label-free absolute quantitative proteomics in human gingival crevicular fluid by LC/MS E (gingival exudatome). J Proteome Res. 2010; 9:2191–99. [PubMed: 20205380]
  16. Silvana P. Barros, Ray Williams, Steven Offenbacher, and Thiago Morelli. Gingival Crevicular as a Source of Biomarkers for Periodontitis. Periodontol 2000. 2016 February; 70(1): 53–64. doi:10.1111/prd.12107.
  17. Lucía Melguizo-Rodríguez, Victor J. Costela-Ruiz Francisco Javier Manzano-Moreno Concepción Ruiz and Rebeca Illescas-Montes. Salivary Biomarkers and Their Application in theDiagnosis and Monitoring of the Most Common Oral Pathologies. Int. J. Mol. Sci. 2020, 21, 1-17.  doi:10.3390/ijms21145173.
  18. Nikhil, Vineeta, Biomarkers: A stepping stone for the future of endodontics. IP Indian Journal of Conservative and Endodontics. 2024:9. 108-110. 10.18231/j.ijce. 2024.024.
  19. Jain A, Aurwade V, Bahuguna R, Bgarwal A. Biomarkers of healthy and diseased pulp and periapical tissue: A review. University J Dent Scie 2020; 6 (2):94-100.
  20. Shin SJ, Lee JI, Baek SH, Lim SS. Tissue levels of matrix metalloproteinases in pulps and periapical lesions. Journal of Endodontics. 2002 Apr 1;28(4):313-15.
  21. Aguirre-López EC, Patiño-Marín N, Martínez-Castañón GA, Medina-Solís CE, Castillo-Silva BE, Cepeda- Argüelles O, Aguilera-Galaviz LA, Rosales-García P. Levels of matrix metalloproteinase-8 and cold test in reversible and irreversible pulpitis. Medicine. 2020 Dec 24;99(52):e23782.
  22. Sharma R, Kumar V, Logani A, Chawla A, Mir RA, Sharma S, Kalaivani M. Association between concentration of active MMP‐9 in pulpal blood and pulpotomy outcome in permanent mature teeth with irreversible pulpitis–a preliminary study. International Endodontic Journal. 2021 Apr;54(4):479-89.
  23. Abd-Elmeguid A, Abdeldayem M, Kline LW, Moqbel R, Vliagoftis H, Donald CY. Osteocalcin expression in pulp inflammation. Journal of Endodontics. 2013 Jul 1;39(7): 865-72.
  24. Kritikou K, Greabu M, Imre M, Miricescu D, RipszkyTotan A, Burcea M, Stanescu-Spinu II, Spinu T. ILs and MMPs levels in inflamed human dental pulp: a systematic review. Molecules. 2021 Jul 7;26(14):4129.
  25. Elsalhy M, Azizieh F, Raghupathy R. Cytokines as diagnostic markers of pulpal inflammation. International endodontic journal. 2013 Jun;46(6):573-80.
  26. Salinas-Muñoz M, Garrido-Flores M, Baeza M, Huamán- Chipana P, García-Sesnich J, Bologna R, Vernal R, Hernández M. Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin. Clinical oral investigations. 2017 Nov;21:2613-38.
  27. Mona M, Abbasi Z, Kobeissy F, Chahbandar A, Pileggi R. A bioinformatics systems biology analysis of the current oral proteomic biomarkers and implications for diagnosis and treatment of external root resorption. International journal of molecular sciences. 2021 Mar 20;22(6):3181.
  28. Zhang Y, Guo J, Jia R. Treg: a promising immunotherapeutic target in oral diseases. Frontiers in Immunology. 2021 Jun 10; 12:667862.
  29. Liao J, Al Shahrani M, Al-Habib M, Tanaka T, Huang GT. Cells isolated from inflamed periapical tissue express mesenchymal stem cell markers and are highly osteogenic. Journal of endodontics. 2011 Sep 1;37(9):1217-24.
  30. Emmanuel J. Favaloro , Georgia McCaughan, Leonardo Pasalic. Clinical and laboratory diagnosis of heparin induced thrombocytopenia: an update. Pathology.2017;49(4):346-55.
  31. Shagun Gupta, Kritika Ramesh, Suhaib Ahmed, Vipan Kakkar. Lab-on-Chip Technology: A Review on Design Trends and Future Scope in Biomedical Applications. International journal of bio sciences and Bio Technology. 2016:8(5);311-22.
  32. Melika Haji Mohammadi, Sterre Mulder 2, Patricia Khashayar, Alireza Kalbasi , Mostafa Azimzadeh, Amir Reza Aref. Saliva Lab-on-a-chip Biosensors:recent novel ideas and applications in disease detection. Microchemical Journal. 2021.106506
  33. Andres Escobar, Phyllis Chiu, Jianxi Qu, Yushan Zhang, Chang-qing Xu. Integrated Microfluidic-Based Platforms for On-Site Detection and Quantification of Infectious Pathogens: Towards On-Site Medical Translation of SARS-CoV-2 Diagnostic Platforms. Micromachines 2021, 12, 1079.
  34. Amy e. Herra, anson v. Hatcha, william v. Giannobileb, daniel j. Throckmortona, huu m. Trana, james s. Brennana, and anup k. Singha. Integrated Microfluidic Platform for Oral Diagnostics. 2007 March ; 1098: 362–374. doi:10.1196/annals.1384.004.

For evaluating the accuracy of vital pulp therapy for an inflamed pulp, the use of non-invasive diagnostic tools are essential to avoid further damage to the pulp dentin complex. The identification of specific biomarkers reflecting the inflammatory status of the dental pulp holds significant promise in guiding accurate diagnosis and enabling personalized treatment strategies. This review article focuses upon delivering brief information on endodontic biomarkers seen in different pulpal and peri apical pathologies and their isolation techniques.

Keywords : Endodontic Biomarkers, Periapical Inflammation, Apical Periodontitis, Diagnostic Test.

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Paper Submission Last Date
31 - December - 2025

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