Enhanced Accumulation of Lead and Copper in J. curcas using Ethylene diamine-tetra-acetic acid (EDTA)


Authors : Dayo Lateefat Yusuf; Samaila Muazu Batagarawa; Ibrahim Sada; Nasiru Hassan Wagini

Volume/Issue : Volume 8 - 2023, Issue 8 - August

Google Scholar : https://bit.ly/3TmGbDi

Scribd : https://tinyurl.com/yf3w4x7c

DOI : https://doi.org/10.5281/zenodo.8275721

Abstract : In this study, the ability of J. curcas in the extraction of Lead (Pb) and Copper (Cu) from soils irrigated with municipal waste water was investigated. The seeds of J. curcas were planted in pots containing soil (1kg) obtained from vegetable garden irrigated with municipal waste water. Due to the limited availability of some metals to plants, chelating agents such as ethylene diamine tetra acetic acid (EDTA) was applied to the soil to facilitate the solubilisation and transport of these metals to the plant roots. EDTA (5ml, 0.1mmol) was added on the 10th, 11th and 12th week. In the control, EDTA was not added and all the plants were harvested after 13 weeks of planting. The average number of leaves and shoot heights of the plants were recorded. Pb and Cu contents in the roots and shoots of the plants in EDTA treated soil and control were analyzed using Microwave plasma atomic emission spectrophotometer (MP- AES). The results obtained showed that the application of EDTA increased (P < 0.05) the average number of leaves and shoot heights of J. curcas when compared to control. The results obtained also showed that EDTA increased (P < 0.05) the uptake of metals in the tissues of the plant when compared to control. Phytoextraction ability was assessed in terms of bio concentration factor (BCF) and transfer factor (TF). Based on the TF and BCF values, J. curcas can be used as a good candidate for the removal of Pb and Cu in the soil.

Keywords : Phytoremediation, Municipal Waste Water, Vegetable Garden, Edta.

In this study, the ability of J. curcas in the extraction of Lead (Pb) and Copper (Cu) from soils irrigated with municipal waste water was investigated. The seeds of J. curcas were planted in pots containing soil (1kg) obtained from vegetable garden irrigated with municipal waste water. Due to the limited availability of some metals to plants, chelating agents such as ethylene diamine tetra acetic acid (EDTA) was applied to the soil to facilitate the solubilisation and transport of these metals to the plant roots. EDTA (5ml, 0.1mmol) was added on the 10th, 11th and 12th week. In the control, EDTA was not added and all the plants were harvested after 13 weeks of planting. The average number of leaves and shoot heights of the plants were recorded. Pb and Cu contents in the roots and shoots of the plants in EDTA treated soil and control were analyzed using Microwave plasma atomic emission spectrophotometer (MP- AES). The results obtained showed that the application of EDTA increased (P < 0.05) the average number of leaves and shoot heights of J. curcas when compared to control. The results obtained also showed that EDTA increased (P < 0.05) the uptake of metals in the tissues of the plant when compared to control. Phytoextraction ability was assessed in terms of bio concentration factor (BCF) and transfer factor (TF). Based on the TF and BCF values, J. curcas can be used as a good candidate for the removal of Pb and Cu in the soil.

Keywords : Phytoremediation, Municipal Waste Water, Vegetable Garden, Edta.

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