Authors :
Exsyupransia Mursyant; Stefani Santi Widhiastuti
Volume/Issue :
Volume 9 - 2024, Issue 3 - March
Google Scholar :
https://tinyurl.com/mth2n83t
Scribd :
https://tinyurl.com/mrxj8ykj
DOI :
https://doi.org/10.38124/ijisrt/IJISRT24MAR149
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
The propagation of Clitoria ternatea poses
several challenges, including low germination rates,
vulnerability of young seedlings in natural environment,
and a slow flowering rate. This study aims to identify the
best Plant Growth Regulators and their optimal
concentration to stimulate indirect somatic
embryogenesis from C. Ternatea leaves explants. The
samples were cultured on Murashige and Skoog (MS)
medium supplemented with various concentration of
thidiazuron (TDZ; 1, 2, 3, 4, and 5 mgL-1
) and a
combination of 2,4-dichloro phenoxy acetic acid (2,4-D; 1,
2, and 3 mgL-1
) and 0.1 mgL-1 kinetin. The findings
revealed that all treatments resulted in the formation of
both embryogenic and non embryogenic calluses. The
addition of TDZ to the MS medium led to a greater
percentage of embryogenic callus formation compared to
using the combination of kinetin and 2,4-D. The highest
percentage of 84.37% was achieved when samples were
cultured on MS medium with 3 mgL-1 TDZ. The most
effective induction period of 21 days was observed with
the medium supplemented with 3 mgL-1 TDZ, followed by
4 and 5 mgL-1 TDZ, which took 23 days. The research
findings suggest that a concentration of 3 mgL-1 TDZ is
the optimal PGR type and dosage for achieving rapid and
efficient in vitro plant production compared to various
concentrations of 2,4-D in combination with kinetin.
Keywords :
Butterfly Pea, Dichlorophenoxyacetic Acid, Embryogenesis, Thidiazuron.
The propagation of Clitoria ternatea poses
several challenges, including low germination rates,
vulnerability of young seedlings in natural environment,
and a slow flowering rate. This study aims to identify the
best Plant Growth Regulators and their optimal
concentration to stimulate indirect somatic
embryogenesis from C. Ternatea leaves explants. The
samples were cultured on Murashige and Skoog (MS)
medium supplemented with various concentration of
thidiazuron (TDZ; 1, 2, 3, 4, and 5 mgL-1
) and a
combination of 2,4-dichloro phenoxy acetic acid (2,4-D; 1,
2, and 3 mgL-1
) and 0.1 mgL-1 kinetin. The findings
revealed that all treatments resulted in the formation of
both embryogenic and non embryogenic calluses. The
addition of TDZ to the MS medium led to a greater
percentage of embryogenic callus formation compared to
using the combination of kinetin and 2,4-D. The highest
percentage of 84.37% was achieved when samples were
cultured on MS medium with 3 mgL-1 TDZ. The most
effective induction period of 21 days was observed with
the medium supplemented with 3 mgL-1 TDZ, followed by
4 and 5 mgL-1 TDZ, which took 23 days. The research
findings suggest that a concentration of 3 mgL-1 TDZ is
the optimal PGR type and dosage for achieving rapid and
efficient in vitro plant production compared to various
concentrations of 2,4-D in combination with kinetin.
Keywords :
Butterfly Pea, Dichlorophenoxyacetic Acid, Embryogenesis, Thidiazuron.
