Authors :
Dr. W.D.Sam Solomon; Priyadharshini.R; K.Hemamalini; V.Karnan; S.Priyavagini; K.R.Anjana
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/3Gp1uio
DOI :
https://doi.org/10.5281/zenodo.7807241
Abstract :
A rapid, accurate and precise HPTLC
method has been developed for the estimation of
Piracetam in bulk and Pharmaceutical formulation. In
this method standard and sample solutions of Piracetam
were applied on pre-coated 6 x 10 silica gel 60F254 TLC
plate, and developed using chloroform: methanol:glacial
acetic acid: triethyl amine (16:4:0.2:0.2) as mobile phase.
A Camag HPTLC system comprising of Camag Linomat
-5-applicator, Camag twin trough chamber, Camag
TLC-3 scanner was used for the analysis. The drugs on
the plate were scanned at 254 nm. The dynamic linearity
range was 2µl, 4µl, 6µl, 8µl & 10µl for Piracetam. The
method was validated for precision, accuracy and
recovery and the results were satisfactory.Precision
(%RSD-0.31), Accuracy (%RSD-0.59) values are under
the limit. According to ICH guidelines the values of
accuracy and precision should be in the limit less than 2.
Keywords :
Estimation, HPTLC, Piracetam
A rapid, accurate and precise HPTLC
method has been developed for the estimation of
Piracetam in bulk and Pharmaceutical formulation. In
this method standard and sample solutions of Piracetam
were applied on pre-coated 6 x 10 silica gel 60F254 TLC
plate, and developed using chloroform: methanol:glacial
acetic acid: triethyl amine (16:4:0.2:0.2) as mobile phase.
A Camag HPTLC system comprising of Camag Linomat
-5-applicator, Camag twin trough chamber, Camag
TLC-3 scanner was used for the analysis. The drugs on
the plate were scanned at 254 nm. The dynamic linearity
range was 2µl, 4µl, 6µl, 8µl & 10µl for Piracetam. The
method was validated for precision, accuracy and
recovery and the results were satisfactory.Precision
(%RSD-0.31), Accuracy (%RSD-0.59) values are under
the limit. According to ICH guidelines the values of
accuracy and precision should be in the limit less than 2.
Keywords :
Estimation, HPTLC, Piracetam
