Evaluation of Bioethanol Quality Produced by Sweet Sorghum Stalks in Uganda


Authors : Atem David; Lating; Betty Nabuuma; Steven; Alexander Bombom; Wembazi Enock; Babirye Fatumah; Nuwamanya Ephrai; Samuel Mulondo; Byansi Anjero

Volume/Issue : Volume 10 - 2025, Issue 7 - July


Google Scholar : https://tinyurl.com/akjur959

Scribd : https://tinyurl.com/yd932hrz

DOI : https://doi.org/10.38124/ijisrt/25jul914

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Note : Google Scholar may take 30 to 40 days to display the article.


Abstract : Sweet sorghum (Sorghum bicolor) is a drought-tolerant plant with high sugar content and low input requirements, making it a promising candidate for bioethanol production. This study aimed to assess bioethanol production from local sweet sorghum varieties in Uganda. Six varieties with brix content ranging from 10% to 25% were screened, and juice was extracted and fermented using the SC yeast strain for 10 days, followed by fractional distillation to produce bioethanol. The feedstock was characterized by protein, reducing sugars, and carbohydrate content. Using response surface methodology (RSM) and a central composite design, 20 experimental runs were conducted to optimize yeast loading, reaction time, and agitation rate. The optimal parameters identified were 25 g of yeast loading, 10 days of reaction time, and an agitation rate of 100 rpm. The resulting bioethanol concentrations from the sweet sorghum juice ranged from 56 % v/v to 90% v/v, while concentrations from the bagasse ranged from 15% v/v to 40% v/v. The higher heating value (HHV) of the bioethanol produced varied from 12.46 MJ/kg to 16.79 MJ/kg. Quality assessments using bomb calorimetry, density pycnometry, and fire and flash point tests revealed HHVs between 13.06 MJ/kg and 20.31 MJ/kg, juice densities of 0.82 g/cm3 to 0.87 g/cm3, bagasse densities of 0.90 g/cm3 to 0.96 g/cm3, and flash points ranging from 17.8°C to 23.0°C for juice and 25.5°C to 45.3°C for bagasse. This study demonstrates that local sweet sorghum stalks have significant potential for bioethanol production, offering a sustainable alternative to fossil fuels in developing countries.

References :

  1. Adinurani, P., Rahayu, S., Sulistiyo Budi, L., Nindita, A., Soni, P., & Mel, M. (2018). Biomass and Sugar Content of Some Varieties of Sorghum (Sorghum Bicolor L. Moench) on Dry Land Forest as Feedstock Bioethanol. MATEC Web of Conferences, 164, 1–5. https://doi.org/10.1051/matecconf/201816401035
  2. Assaye, M., Tamirat, B., & Fekadu, B. (2021). Ethanol concentration and calorific value of some local distilled Ethiopian alcohol ( Areki ): an energy potential assessment Ethanol concentration and calorific value of some local distilled Ethiopian alcohol ( Areki ): an energy potential assessment. Cogent Engineering, 8(1). https://doi.org/10.1080/23311916.2021.1979444
  3. Azhar, S. H., Abdulla, R., Jambo, S. A., Marbawi, H., Gansau, J. A., Mohd Faik, A. A., & Rodrigues, K. F. (2017). Yeasts in sustainable bioethanol production. Elsevier, 10(March), 52–61. https://doi.org/10.1016/j.bbrep.2017.03.003
  4. Barcelos, C. A., Maeda, R. N., Maria, L., Anna, M. S., & Pereira, N. (2016). Biomass and Bioenergy Sweet sorghum as a whole-crop feedstock for ethanol production. Elsevier, 94, 46–56.
  5. Brakenhoff, D. A., Vonk, M. A., Collenteur, R. A., & Baar, M. Van. (2022). Application of Time Series Analysis to Estimate Drawdown From Multiple Well Fields. Frontiers, 10(June), 1–13. https://doi.org/10.3389/feart.2022.907609
  6. Byun, J., & Han, J. (2021). Environmental analysis of bioethanol production strategies from corn stover via enzymatic and nonenzymatic sugar production. Elsevier, 328(February), 124808. https://doi.org/10.1016/j.biortech.2021.124808
  7. Carareto, N. D. D., Kimura, C. Y. C. S., Oliveira, E. C., Costa, M. C., & Meirelles, A. J. A. (2012). Flash points of mixtures containing ethyl esters or ethylic biodiesel and ethanol. Elsevier, 96, 319–326. https://doi.org/10.1016/j.fuel.2012.01.025
  8. Cristina, B., Gheorghe, M., & Maria, D. A. (2017). Evaluation of Sugar Content in Stem Juice From Sweet Sorghum Hybrids Grown in South West of Romania. Cadastre Series), Anglani 1998, 25–29.
  9. Frankowski, J., Wawro, A., Batog, J., Szambelan, K., & Łacka, A. (2022). Bioethanol Production Efficiency from Sorghum Waste Biomass. Energies, 15(9), 1–11. https://doi.org/10.3390/en15093132
  10. Holechek. (2022). A Global Assessment: Can Renewable Energy Replace Fossil Fuels by 2050? MDPI, 14(8), 1–22. https://doi.org/10.3390/su14084792
  11. Janes, A., & Chaineaux, J. (2014). Experimental determination of flash points of flammable liquid aqueous solutions To cite this version : HAL Id : ineris-00976242 Experimental Determination of Flash Points of Flammable Liquid Aqueous Solutions. Hall Open Science.
  12. Jeswani, H. K., Chilvers, A., & Azapagic, A. (2020). Environmental sustainability of biofuels: A review: Environmental sustainability of biofuels. Royal Academy of Engineering, 476(2243). https://doi.org/10.1098/rspa.2020.0351
  13. Kasegn, M. M. (2023). Production of Bioethanol from Sweet Sorghum [ Sorghum Bicolor L .] Juice Using Yeast Isolated from Fermented Sweet Sorghum Juice. Research Square, 1–12.
  14. Khattab, I. S., Bandarkar, F., Amin, M., Fakhree, A., & Jouyban, A. (2012). Density , viscosity , and surface tension of water + ethanol mixtures from 293 to 323 K. Korean Journal of Chemical Engineering, 29(6), 812–817. https://doi.org/10.1007/s11814-011-0239-6
  15. Kumari, M., & Gupta, S. K. (2019). Response surface methodological (RSM) approach for optimizing the removal of trihalomethanes (THMs) and its precursor’s by surfactant modified magnetic nanoadsorbents (sMNP) - An endeavor to diminish probable cancer risk. Scientific Reports, 9(1), 1–11. https://doi.org/10.1038/s41598-019-54902-8
  16. López-Sandin, I., Gutiérrez-Soto, G., Gutiérrez-Díez, A., Medina-Herrera, N., Gutiérrez-Castorena, E., Galicia-Juárez, M., & Zavala-García, F. (2021). Biomass and sugar production dynamics in sweet sorghum variety roger. Chilean Journal of Agricultural Research, 81(1), 92–101. https://doi.org/10.4067/S0718-58392021000100092
  17. Micic, V., & Jotanovic, M. (2015). Bioethanol as fuel for internal combustion engines. Zastita Materijala, 56(4), 403–408. https://doi.org/10.5937/zasmat1504403m
  18. Muhaji, & Sutjahjo, D. H. (2018). The characteristics of bioethanol fuel made of vegetable raw materials. IOP Conference Series: Materials Science and Engineering, 296(1). https://doi.org/10.1088/1757-899X/296/1/012019
  19. Nuanpeng, S., Thanonkeo, S., Klanrit, P., & Yamada, M. (2023). Optimization Conditions for Ethanol Production from Sweet Sorghum Juice by Thermotolerant Yeast Saccharomyces cerevisiae : Using a Statistical Experimental Design. MDPI, 04, 1–15.
  20. Pappu, J. S. M., & Gummadi, S. N. (2016). Multi response optimization for enhanced xylitol production by Debaryomyces nepalensis in bioreactor. Springer, 6(2), 1–10. https://doi.org/10.1007/s13205-016-0467-x
  21. Phukoetphim, N., Salakkam, A., Laopaiboon, P., & Laopaiboon, L. (2017). Improvement of ethanol production from sweet sorghum juice under batch and fed-batch fermentations: Effects of sugar levels, nitrogen supplementation, and feeding regimes. Electronic Journal of Biotechnology, 26, 84–92. https://doi.org/10.1016/j.ejbt.2017.01.005
  22. Rajewski, J., & Dobrzyńska-Inger, A. (2021). Application of response surface methodology (Rsm) for the optimization of chromium(iii) synergistic extraction by supported liquid membrane. MDPI, 11(11), 1–17. https://doi.org/10.3390/membranes11110854
  23. Saini, J. K., Anurag, R. K., Arya, A., Kumbhar, B. K., & Tewari, L. (2013). Optimization of saccharification of sweet sorghum bagasse using response surface methodology. Industrial Crops and Products, 44, 211–219. https://doi.org/10.1016/j.indcrop.2012.11.011
  24. Yaser Dahman, Kashif Syed, Sarkar Begum, Pallavi Roy,  and B. M. D. (2019). Biofuels: Their characteristics and analysis. In Elsevier. Elsevier Ltd. https://doi.org/10.1016/B978-0-08-102426-3.00014-X

Sweet sorghum (Sorghum bicolor) is a drought-tolerant plant with high sugar content and low input requirements, making it a promising candidate for bioethanol production. This study aimed to assess bioethanol production from local sweet sorghum varieties in Uganda. Six varieties with brix content ranging from 10% to 25% were screened, and juice was extracted and fermented using the SC yeast strain for 10 days, followed by fractional distillation to produce bioethanol. The feedstock was characterized by protein, reducing sugars, and carbohydrate content. Using response surface methodology (RSM) and a central composite design, 20 experimental runs were conducted to optimize yeast loading, reaction time, and agitation rate. The optimal parameters identified were 25 g of yeast loading, 10 days of reaction time, and an agitation rate of 100 rpm. The resulting bioethanol concentrations from the sweet sorghum juice ranged from 56 % v/v to 90% v/v, while concentrations from the bagasse ranged from 15% v/v to 40% v/v. The higher heating value (HHV) of the bioethanol produced varied from 12.46 MJ/kg to 16.79 MJ/kg. Quality assessments using bomb calorimetry, density pycnometry, and fire and flash point tests revealed HHVs between 13.06 MJ/kg and 20.31 MJ/kg, juice densities of 0.82 g/cm3 to 0.87 g/cm3, bagasse densities of 0.90 g/cm3 to 0.96 g/cm3, and flash points ranging from 17.8°C to 23.0°C for juice and 25.5°C to 45.3°C for bagasse. This study demonstrates that local sweet sorghum stalks have significant potential for bioethanol production, offering a sustainable alternative to fossil fuels in developing countries.

CALL FOR PAPERS


Paper Submission Last Date
31 - December - 2025

Video Explanation for Published paper

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe