Authors :
Purabi Saha; Anubhav Dubey; Dr. Sanjay Kumar; Roshan Kumar
Volume/Issue :
Volume 7 - 2022, Issue 5 - May
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3x7J0hX
DOI :
https://doi.org/10.5281/zenodo.6604903
Abstract :
Antimicrobial-resistant microbes are now
infecting doctors, veterinarians, and other infection
control specialists.
Material & Method: Collection of samples and isolation
of K. pneumoniae, Anti-microbial Susceptibility pattern,
Hodge's modified test, Extraction of the bla KPC2 gene's
DNA
Result & Discussion: More than half of the isolates were
resistant to both imipenem and meropenem, with four
being just resistant to imipenem. Among the 38
carbapenem resistant isolates, 71% had imipenem MICs
above CLSI norms, whereas the remaining 79.9% were
intermediately resistant. The MIC50 and MIC90 were
0.19-32, 4 and 16 g/ml, respectively (21.05 percent ). Eight
of the isolates had MHT. The blaKPC-2 imipenem MIC is
4-32 g/ml. Neither colistin nor co-trimaxazole resistant
BlaKPC-2 isolates.
Conclusion: Our institution found blaKPC-2 to be a
carbapenem resistant K. pneumoniae pathway. Results
demonstrated imipenem disc diffusion can quickly
identify these isolates. These illnesses and their associated
drug resistance demand infection control and antibiotic
stewardship measures.
Keywords :
K. Pneumonia, gene, DNA, Anti-microbial.
Antimicrobial-resistant microbes are now
infecting doctors, veterinarians, and other infection
control specialists.
Material & Method: Collection of samples and isolation
of K. pneumoniae, Anti-microbial Susceptibility pattern,
Hodge's modified test, Extraction of the bla KPC2 gene's
DNA
Result & Discussion: More than half of the isolates were
resistant to both imipenem and meropenem, with four
being just resistant to imipenem. Among the 38
carbapenem resistant isolates, 71% had imipenem MICs
above CLSI norms, whereas the remaining 79.9% were
intermediately resistant. The MIC50 and MIC90 were
0.19-32, 4 and 16 g/ml, respectively (21.05 percent ). Eight
of the isolates had MHT. The blaKPC-2 imipenem MIC is
4-32 g/ml. Neither colistin nor co-trimaxazole resistant
BlaKPC-2 isolates.
Conclusion: Our institution found blaKPC-2 to be a
carbapenem resistant K. pneumoniae pathway. Results
demonstrated imipenem disc diffusion can quickly
identify these isolates. These illnesses and their associated
drug resistance demand infection control and antibiotic
stewardship measures.
Keywords :
K. Pneumonia, gene, DNA, Anti-microbial.
