Authors :
Ngari P. Muriuk; Ithinji G. D; Leonard Ateya; Caroline Wasonga; Joel Lutomiah; Muthamia M. Kiraithe
Volume/Issue :
Volume 8 - 2023, Issue 5 - May
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/3CqMUnW
DOI :
https://doi.org/10.5281/zenodo.8023100
Abstract :
Sheep and goats (n=4/group) were inoculated
with thermos-stabilized inactivated Nairobi sheep
disease virus vaccine. Four unvaccinated animals for
each group were kept as control. Vaccinated groups
were given a booster vaccine dose 21 days later. Immune
response was monitored by neutralizing antibody titers
were determined by micro-plaque reduction
neutralization test and confirmed by
immunofluorescence assay. Two doses of the inactivated
vaccine stimulated a strong immune response in the
vaccinated animals. The vaccinated and mock group
were challenged with virulent 1473 strain of the Nairobi
sheep disease virus. All animals developed fever and
viremia with varying degrees between sheep and goats
post challenge. Mock vaccinated sheep developed high
viremia levels relative to the vaccinated group and
developed severe disease. In contrast, mock vaccinated
goats showed a slight temperature compared to
vaccinated goats. After challenge, two control sheep died
from the disease whereas the vaccinated sheep survived.
Vaccinated sheep suffered mild to moderate clinical
reactions with pyrexia. Formalin inactivated vaccine
fully protected the animals against the lethal 1473
challenge virus.
Sheep and goats (n=4/group) were inoculated
with thermos-stabilized inactivated Nairobi sheep
disease virus vaccine. Four unvaccinated animals for
each group were kept as control. Vaccinated groups
were given a booster vaccine dose 21 days later. Immune
response was monitored by neutralizing antibody titers
were determined by micro-plaque reduction
neutralization test and confirmed by
immunofluorescence assay. Two doses of the inactivated
vaccine stimulated a strong immune response in the
vaccinated animals. The vaccinated and mock group
were challenged with virulent 1473 strain of the Nairobi
sheep disease virus. All animals developed fever and
viremia with varying degrees between sheep and goats
post challenge. Mock vaccinated sheep developed high
viremia levels relative to the vaccinated group and
developed severe disease. In contrast, mock vaccinated
goats showed a slight temperature compared to
vaccinated goats. After challenge, two control sheep died
from the disease whereas the vaccinated sheep survived.
Vaccinated sheep suffered mild to moderate clinical
reactions with pyrexia. Formalin inactivated vaccine
fully protected the animals against the lethal 1473
challenge virus.
