Expression Analysis of Poly (ADP-Ribose) Polymerase1 Gene in Workers of Salt Mines

Authors : Saffia Shaheen; Zertashia Akram; Tehmina Mezhar; Ishrat Mahjabeen; Muhammad Rizwan

Volume/Issue : Volume 6 - 2021, Issue 12 - December

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Poly (ADP-Ribose) Polymerase1, PARP1 is an important protein coding gene that involve in DNA repair mechanism. It acts as a first responder that detects DNA damage and then facilitates choice for repair pathways. The current study was design to study the expression variation of PARP1 gene in salt mine workers. In the present study 200 blood samples of exposed salt mine workers along with 200 control groups were used. Expression variety of PARP1 quality was assessed by qPCR. Relative expression of PARP1 was (p<0.01) up regulated in exposed groups compared with control groups. Relative expression of PARP1 gene increased significantly in worker group with >30-year age compared to their respective controls (P<0.01) and even to worker group with <30-year age (P<0.05). The expression of PARP1 gene seen unregulated in mine who work in mine more than 10 years. Study cohort of workers and control group was divided as smokers and non-smokers. Significant (P<0.05) up regulation in PARP1 expression was seen in smoker category of mine workers compared with control smokers and nonsmokers of mine workers. Mine workers divided into groups based on type of fuel used by workers performing duty as workers using gas or wood. Significant (P<0.05) up regulation of PARP1 gene was observed in mine group working with wood compared to group working with gas. The obtained results suggest that salt mine workers are exposed to different genotoxins at their workplaces which affect the genetic integrity and DNA repair capacity.


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