The fresh water biodiversity is an unpredictable for natural product research from northern region, India, Barytelphusa cunicularis is major species, the Ethanol crude extract of tissue has been utilized for this study the isolation, separation and Characterization by using reputed methods. The biological activity of the crude ethanol extract was examined then this fresh water crab was resulted antibacterial and antifungal activity it means the bioactive compounds have been present in this crab. TLC was performed to set the solvent system for column chromatography while purity level to be checked by HPLC, it is 93% pure compound.

Keywords : Barytelphusa Cunicularis, Freshwater Crab, Unpredictable, Bioactive Compounds, Crude Extract.