The aim of this study was to exterminate the factors influencing the use of Prevention of Mother-ToChild Transmission of HIV (PMTCT) Services in Ilala District Dar es salaam Region. Interviews, questionnaire, and documentary reviews were used for data collection. The study used quantitative approach to analyse data mathematically. The study found that age, sex, and marital status were influencing the use of PMTCT services. Other factors included limited support from partners in the use of PMTCT services and education levels of women, level of income and stigmatization. This study was conducted after a determination that there are limiting number of documented studies which has been published on the socio-economic demographic and cultural factors influencing the use of PMTCT of HIV services in Ilala district where about 49,823 expectant mothers were tested for HIV/AIDS status and the status of their children born in 2018 and the results indicated that 2.7% were HIV/AIDS positive. The study recommends that the government through its sectoral ministries and partner agencies should strengthening community awareness on HIV, engaging male involvement in PMTCT care, and getting friendly service for the better PMTCT service utilization

Keywords : Prevention, Mother to child transmission, HIV/AIDS, Tanzania.