Herbs are obtained from natural sources.
Now a days they are being incorporated into
cosmeceutical formulations due to the presence of
various properties like anti-bacterial, anti-viral, anti-
oxidant and anti-inflammatory activities. The goal of the
present study was to formulate and evaluate herbal
lipstick, screening of phytochemicals and to provide in-
vitro evidence for radical scavenging activity of
Ethanolic flower extract of Brassica Oleraceae var.
Italica, characterization of bioactive compound by
chromatographic techniques and its quantification using
analytical technique. Phyto-chemical screening of extract
was performed which indicated the presence of many
active constituents. Lipstick was formulated into five
different formulations and evaluation tests were
performed. The evaluation test results shown that fifth
formulation has good stability than others when
compared to marketed product. The broccoli extract had
shown good antioxidant activity when compared with
standard ascorbic acid. The total phenolic content of
broccoli extract was determined and expressed in gallic
acid equivalents (36.2 ± 0.9). TLC was performed using
quercetin as standard. The bioactive component was
detected as quercetin based on the proximity of Rf value
of sample extract and Rf value of standard quercetin. A
simple, rapid and sensitive method was developed for
HPTLC quantitative analysis and results shown that
100mg of broccoli extract contains 29.6mg of quercetin.
The herbal lipstick BLISS, formulated by us using
broccoli flower extract containing quercetin, a flavanoid
which being an antioxidant, reduces oxidative stress and
prevents photoaging of lips keeping it supple and
luscious in the long run.
Keywords : Broccoli, Lipstick, Photoageing, Antioxidant activity, Quercetin, HPTLC Analysis.