Authors : Pushkar Bhalchandra Karande Patil; Madan Dadasaheb Pomaje; Dr. Ashwini. B. Patil

Volume/Issue : Volume 11 - 2026, Issue 1 - January

Google Scholar : https://tinyurl.com/4ut5spn3

Scribd : https://tinyurl.com/yd4ayeea

DOI : https://doi.org/10.38124/ijisrt/26jan996

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : This study developed and evaluated a polyherbal anti-asthmatic cough syrup using Glycyrrhiza glabra, Withania somnifera, Cinnamomum verum and Bauhinia racemosa. These plants were chosen for their documented anti-asthmatic, anti-inflammatory, mucolytic, antioxidant, and antimicrobial effects. Extraction was carried out by Soxhlet method with ethanol for Withania somnifera and Cinnamomum verum, while Bauhinia racemosa and Glycyrrhiza glabra were extracted by cold maceration using ethanol and distilled water, respectively. The extracts were concentrated into semi-solid residues for formulation. Phytochemical analysis confirmed the presence of Quercetin in Bauhinia racemosa, Glycyrrhizin in Glycyrrhiza glabra, and Cinnamaldehyde in Cinnamomum verum. UV-visible spectroscopy verified extract purity, with maximum absorbance values matching reported standards and calibration curves showing strong linearity, confirming reliability of the method. FTIR spectroscopy and DSC indicated no significant interaction between extracts and excipients, establishing stability of the formulation. Three formulations were developed and tested, among which the third formulation was most compliant with Indian Pharmacopoeial standards. Each 10 milliliters contained Bauhinia racemosa 100 milligrams, Cinnamomum verum 100 milligrams, Withania somnifera 200 milligrams, and Glycyrrhiza glabra 200 milligrams in a sugar syrup base with preservatives. The formulation showed significant pharmacological activity. Anti-inflammatory testing revealed 65 to 74 percent inhibition, with Glycyrrhiza glabra reaching 87.4 percent, comparable to diclofenac sodium. Antioxidant activity was highest in Withania somnifera at 88.3 percent, while the syrup demonstrated 59 to 65 percent inhibition. Antibacterial testing showed Cinnamomum verum with the strongest effect at 18.7 millimeters of inhibition, followed by Bauhinia racemosa at 9.8 millimeters, and the formulation at 8.2 millimeters against Klebsiella pneumoniae. Ex- vivo studies on goat trachea showed that the syrup inhibited 77.3 percent of histamine-induced contraction, confirming bronchodilatory and antihistaminic potential. Overall, the formulation demonstrated strong anti-inflammatory, antioxidant, antimicrobial, and bronchodilatory effects, supporting its use as an anti-asthmatic cough syrup.

Keywords : Polyherbal Formulation, Antiasthmatic, Bauhinia racemosa, Cinnamomum verum, Withania somnifera, Glycyrrhiza glabra.

References :

1. Boers, E.; Barrett, M.; Su, J. G.; Benjafield, A. V.; Sinha, S.; Kaye, L.; Zar, H. J.; Vuong, V.; Tellez, D.; Gondalia, R.; Rice, M. B.; Nunez, C. M.; Wedzicha, J. A.; Malhotra, A. global burden of chronic obstructive pulmonary disease through 2050. jama netw open 2023, 6 (12), e2346598. https://doi.org/10.1001/jamanetworkopen.2023.46598.
2. Umamaheswari, D.; Arun, V.; Jeeva, K.; Venkateswarlu, B. S.; Kumar, M. Comparative evaluation of herbal cough syrup available in market. International journal of research and analytical reviews 2020.
3. Prabhakar Bangar, A.; Jatale, A.; Deshmukh, S. Poly herbal formulation and evaluation of herbal cough syrup. www.ijirct.org.
4. Sharma, V.; Singh, S.; Dixit, A.; Saxena, A. formulation and evaluation of herbal cough syrup from seeds extract of hedge mustard. International journal of research in pharmacy and chemistry 2020, 10 (1). https://doi.org/10.33289/ijrpc.10.1.2020.10(3).
5. Sumedh Sanjay, K. Formulation and evaluation of herbal cough syrup abstract; 2023; vol. 11. www.ijcrt.org.
6. Panda, P.; Sahu, A. formulation and evaluation of herbal cough syrup. asian journal of pharmaceutical research and development 2023, 11 (2), 28–33. https://doi.org/10.22270/ajprd.v11i2.1234.
7. Pranjal Nimba, P.; Tejashree Sudam, Z.; Kanchan Bhausaheb, T.; Rakesh Manik, W.; Pramod Anna, D. Formulation and evaluation of herbal cough syrup; 2023. www.ijnrd.org.
8. Anitha, P.; Ravichandhiran, E.; Krishnan, R. HPTLC fingerprinting analysis of the ethanolic extract of bauhinia racemosa leaves; 2023; vol. 10. www.ejpmr.com.
9. Prabhu, S.; Vijayakumar, S.; Ramasubbu, R.; Praseetha, P. K.; Karthikeyan, K.; Thiyagarajan, G.; Sureshkumar, J.; Prakash, N. Traditional Uses, Phytochemistry and Pharmacology of Bauhinia Racemosa Lam.: A Comprehensive Review. Futur J Pharm Sci 2021, 7 (1). https://doi.org/10.1186/s43094-021-00251-1.
10. Prabhakar Bangar, A.; Jatale, A.; Deshmukh, S. Polyherbal formulation and evaluation of herbal cough syrup. www.ijirct.org.
11. Nasulhah Kasim, N.; Nursyimi Azlina Syed Ismail, S.; Masdar, N.; Ab Hamid, F.; Nawawi, W. Extraction and potential of cinnamon essential oil towards repellency and insecticidal activity; 2014; vol. 4. www.ijsrp.org.
12. Shinde, S.; Balasubramaniam, A. K.; Mulay, V.; Saste, G.; Girme, A.; Hingorani, L. Recent advancements in extraction techniques of ashwagandha (withania somnifera) with insights on phytochemicals, structural significance, pharmacology, and current trends in food applications. acs omega. american chemical society november 7, 2023, pp 40982–41003. https://doi.org/10.1021/acsomega.3c03491.
13. Bashir, A.; Nabi, M.; Tabassum, N.; Afzal, S.; Ayoub, M. An updated review on phytochemistry and molecular targets of withania somnifera (l.) dunal (ashwagandha). frontiers in pharmacology. frontiers media s.a. 2023. https://doi.org/10.3389/fphar.2023.1049334.
14. Pastorino, G.; Cornara, L.; Soares, S.; Rodrigues, F.; Oliveira, M. B. P. P. Liquorice (Glycyrrhiza Glabra): A phytochemical and pharmacological review. phytotherapy research. john wiley and sons ltd december 1, 2018, pp 2323–2339. https://doi.org/10.1002/ptr.6178.
15. Wahab, S.; Annadurai, S.; Abullais, S. S.; Das, G.; Ahmad, W.; Ahmad, M. F.; Kandasamy, G.; Vasudevan, R.; Ali, M. S.; Amir, M. Glycyrrhiza Glabra (Licorice): A comprehensive review on its phytochemistry, biological activities, clinical evidence and toxicology. plants. mdpi december 1, 2021. https://doi.org/10.3390/plants10122751.
16. Balekundri, A.; Shahapuri, A.; Patil, M. Poly-herbal tablet formulation by design expert tool and in vitro anti-lipase activity. futur j pharm sci 2020, 6 (1). https://doi.org/10.1186/s43094-020-00131-0.
17. Prabhakar Bangar, A.; Jatale, A.; Deshmukh, S. Poly herbal formulation and evaluation of herbal cough syrup. www.ijirct.org.
18. Prabhakar Bangar, A.; Jatale, A.; Deshmukh, S. Poly herbal formulation and evaluation of herbal cough syrup. www.ijirct.org.
19. Parvin, M. S.; Das, N.; Jahan, N.; Akhter, M. A.; Nahar, L.; Islam, M. E. Evaluation of in vitro anti-inflammatory and antibacterial potential of crescentia cujete leaves and stem bark pharmacology and toxicology. bmc res notes 2015, 8 (1). https://doi.org/10.1186/s13104-015-1384-5.
20. Chauhan, A.; Semwal, D.; Mishra, S.; Semwal, R. Ayurvedic research and methodology: present status and future strategies. ayu (An international quarterly journal of research in ayurveda) 2015, 36 (4), 364. https://doi.org/10.4103/0974-8520.190699.
21. Nama, N.; Arora, P.; Arora, N.; Jain, H. Formulation and characterization of bauhinia racemosa extract loaded nanoemulsion by box behnken design. journal of pharmaceutical negative results ¦ 13, 2022. https://doi.org/10.47750/pnr.2022.13.s08.648.
22. Bioactivity of active extracts of leaf of Bauhinia racemosa. International journal of clinical chemistry and laboratory medicine 2019, 5 (2). https://doi.org/10.20431/2455-7153.0502004.
23. Nataraj, R.; Chettiyar, B. Phytochemical screening and antimicrobial activity of “cinnamon zeylanicum.” International journal of pharmaceutical research and innovation 2020, 13, 22–33.
24. Noor, S. U.; Faridah, M. Formulation of liquorice root extract (glycyrrhiza glabra l.) as skin whitening cream.
25. Noor, S. U.; Faridah, M. Formulation of liquorice root extract (glycyrrhiza glabra l.) as lozenges.
26. Mhaske, M.; Diliprao, S.; Jagdish, P.; Vilas, S.; Holkar, M.; Uddhav, P.; Gulve, M.; Subhash, A.; Tarade, M.; Santosh, A.; Scholar, U. G.; Professor, A.; Miratai, M. jetir2206735 Journal of emerging technologies and innovative research (jetir) www.jetir.org h297 Formulation and evaluation of oral antiulcer gel from liquorice extract; 2022. www.jetir.org.
27. Keramatian, B.; Ara, L.; Hajimehdipoor, H. Licorice compound traditional syrup: formulation and development of analysis method. Research journal of pharmacognosy 2022, 9 (1), 111–117. https://doi.org/10.22127/rjp.2021.294636.1739.
28. Omkar, M.; Daware, S.; Tijare, R. D.; Ayush, M.; Chavhan, B. Preparation and evaluation of polyherbal cough syrup: a novel approach. www.ijfmr.com.
29. Kapgate, P. R.; Mandade, R. J.; Dhole, Y. R.; Urkuda Gondhale, M.; Ravindra Lanjewar, S.; Purushottam Bawankar, P. Review on polyherbal syrup for various pharmacological activities; 2023; vol. 26. www.ijppr.humanjournals.comwww.ijppr.humanjournals.com.
30. Sakshi Anant Kadam; Seema Dharmaraj Gadade; Anjali Laxman Kalunke; Dr. L. D. Hingane. Formulation and evalution of polyherbal cough syrup. international journal of advanced research in science, communication and technology 2024, 499–509. https://doi.org/10.48175/IJARSCT-22267.
31. Patel, K.; Patel, D. K. Medicinal importance, pharmacological activities, and analytical aspects of hispidulin: a concise report. journal of traditional and complementary medicine. National taiwan university july 1, 2017, pp 360–366. https://doi.org/10.1016/j.jtcme.2016.11.003.
32. Sutherland, E. R. Nocturnal Asthma. Journal of allergy and clinical immunology. mosby inc. 2005, pp 1179–1186. https://doi.org/10.1016/j.jaci.2005.09.028.
33. Lee, K. K.; Davenport, P. W.; Smith, J. A.; Irwin, R. S.; McGarvey, L.; Mazzone, S. B.; Birring, S. S.; Abu Dabrh, A. M.; Altman, K. W.; Barker, A. F.; Blackhall, F.; Bolser, D. C.; Brightling, C.; Chang, A. B.; Davenport, P.; El Solh, A. A.; Escalante, P.; Field, S. K.; Fisher, D.; French, C. T.; Grant, C.; Harding, S. M.; Harnden, A.; Hill, A. T.; Iyer, V.; Kahrilas, P. J.; Kavanagh, J.; Keogh, K. A.; Lai, K.; Lane, A. P.; Lim, K.; Madison, J. M.; Malesker, M. A.; Murad, M. H.; Narasimhan, M.; Newcombe, P.; Oppenheimer, J.; Rubin, B.; Russell, R. J.; Ryu, J. H.; Singh, S.; Smith, M. P.; Tarlo, S. M.; Vertigan, A. E. Global physiology and pathophysiology of cough: part 1: cough phenomenology – chest guideline and expert panel report. chest. elsevier inc. january 1, 2021, pp 282–293. https://doi.org/10.1016/j.chest.2020.08.2086.
34. Solanki, P.; Mohabe, S. issue 6 www.jetir.org (issn-2349-5162); 2024; vol. 11. www.jetir.org.
35. Ruiz-Alcaraz, A. J.; Baquero, L.; Pérez-Munar, P. M.; Oliva-Bolarín, A.; Sánchez-Martínez, M. A.; Ramos-Molina, B.; Núñez-Sánchez, M. A.; Moreno, D. A. In Vitro study of the differential anti-inflammatory activity of dietary phytochemicals upon human macrophage-like cells as a previous step for dietary intervention. int j mol sci 2024, 25 (19). https://doi.org/10.3390/ijms251910728.