Medicinal plants are more crucial in the field of pharmacology as most pharmaceutical industries; it may depend on medicinal plants for their raw materials. Eupatorium Triplinerve Vahl belongs to Asteraceae family, it is also known as “Ayapana” used for various medicinal properties. The present study was carried out to identify the phytoconstituents in the different solvents of the plant leaf extract. In this investigation, Gas Chromatography-Mass Spectrometry GC-MS) screening was performed to evaluate the phytocompounds and their biological activity. The GC-MS analysis provided peaks area 20.4% of 1-ISOPROPYL-2,5-DIMETHOXY- 4-METHYLBENZENE in ethanol leaf extract, 2H-1- BENZOPYRAN-2-ONE, 7-METHOXY (peak area 7.07%) in methanol leaf extract, 3-Oxabicyclo[4.2.0]oct- 5-ene (peak area 19.47%) in acetone leaf extract respectively. The GC-MS profiling of different leaf extracts revealed the presence of bioactive compounds. There are 90 chemical compounds that have new potential sources of medicines for the treatment of various diseases and are responsible for their therapeutic effects. The results show that Eupatorium Triplinerve contains various bioactive compounds and is recommended as a plant of phytopharmaceutical importance.

Keywords : Pharmacology, Phytoconstituents, Ayapana, Phytocompounds.