Genotoxicity of Selected Insecticides in Glycine Max Root Cells by Single Cell Gel Electrophoresis (Comet) Assay

Authors : Manasa. M.S.

Volume/Issue : Volume 3 - 2018, Issue 11 - November

 Objective:- The objective of this research work is to do a comparative study on the genotoxicity of chemical insecticides and bio insecticides in plants by keeping apical root cells of Glycine max as model.  Methods:- Soybean seeds bought from the market located in south Kerala were divided into four sets so that each set contain four seeds. Set 1 and Set 2 treated with same amount of chemical insecticide solutions, Chlorydust and Lara-909 respectively while set 3 treated with Bio insecticide OCTO Plant protector and set 4 was maintained as control. Comet assay was performed in root tip meristems of soybean after seeds germination. Chemical Insecticide solutions were prepared so that it contained their active ingredient at a concentration of 50 ppm, 100 ppm, 150 ppm and 200 ppm.  Result:- The respective study revealed that 200ppm of insecticides treated seeds were unable to germinate. Both bio and chemical insecticides showed increase in genotoxicity with respect to increase in its concentrations. LARA- 909 contain Cypermethrin showed more genotoxicity in this study and Bio insecticide showed DNA damage than chlorydust contain Chlorpyrifos at all concentrations.  Conclusion:- From above results it can be concluded that over use of both chemical and bio insecticides is highly genotoxic to plants.

Keywords : Glycine max, Genotoxicity, Cypermethrin, Chlorpyrifos, Comet assay.


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