The objective of this study is determine the
effect of Eleusinecoracana seed extract on
gastrointestinal tract (GIT) microbiome of Wistar rat
induced with tramadol. Twenty (20) healthy Wistar
albino rats were weighed and allowed to acclimatize for
one week with free access to food pallets and water and
libitum. They were grouped into three (3) groups of four
(4) rats each as group T1, T2 and control. Group T1 and
T2 were administered with 0.1 and 0.2 g of the ethanolic
extract respectively, while control were given distilled
water. The study shows an increase in the weight of
Wistar rat administered with high dosage of the plant
extract when compared to those administered with low
dosage of the extract and also the control group.
Different microbial isolates isolated from the fecal and
GIT of Wistar rats include; Aeromonashydrophyla,
Proteus spp., Pseudomonasaeruginosa,
Citrobacterfreundii, S. spp., Staphylococcusaureus, C.
freundii, and Providencia spp. The study revealed that
the negative control group contains Pseudomonas
aeruginosa with percentage incidence of 28.1%, followed
by A. hydrophyla and Proteus spp., with percentage
incidence of 25 and 21.9% respectively, while
Citrobacterfreundii has the lowest percentage incidence
of 3.1%. While positive group and group administered
with the plant extract had Providenciaspp as the only
organisms present with percentage incidence of
100%.From the study, it was revealed that the extract of
finger millet have effect on the microbiome of the GIT
with a reduction in the population of bacteria colonies.
The population of the bacterial colonies were reduced
drastically on administration of the plant extract and
Keywords : Gastrointestinal tract, colony count, finger millet, antimicrobial.