Authors : Bruce Tavirimirwa; Sikhulile Siziba; Patience Dera; Tendai Dominic Matekenya; Gevious Sisito; Xavier Zhakata; Butholezwi Ngulube

Volume/Issue : Volume 10 - 2025, Issue 8 - August

Google Scholar : https://tinyurl.com/b9sd7rha

Scribd : https://tinyurl.com/53k58evu

DOI : https://doi.org/10.38124/ijisrt/25aug282

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Abstract : Hypochlorous acid (HOCl), a stabilized form of electrolyzed water, has gained attention as a potent, broad- spectrum antimicrobial agent across human and veterinary medicine. This review synthesizes current knowledge on the potential use of HOCl-based products in cattle production systems, with a particular focus on Sub-Saharan Africa. The antimicrobial, anti-inflammatory, and biofilm-disrupting properties of HOCl offer opportunities for improving cattle health, productivity, and biosecurity, while minimizing chemical residues and environmental impact. Documented benefits include reduced pathogen load on infrastructure, improved wound healing, enhanced water and feed hygiene, and possible improvements in animal growth and carcass quality. Despite these advantages, evidence on the efficacy and safety of HOCl in African cattle systems remains limited, particularly under smallholder conditions. Key knowledge gaps include its effects on indigenous breeds, role in maintaining dry-season body condition, and influence on blood and milk metabolites. Additionally, there is a lack of farmer-centred research exploring perceptions, adoption barriers, and practical implementation in resource-limited settings. The review highlights research priorities, including the need for locally relevant clinical trials, safety assessments, cost-benefit analyses, and studies incorporating farmer perspectives. A multidisciplinary approach integrating veterinary science, microbiology, environmental health, and socioeconomics is recommended. Overall, HOCl represents a promising, environmentally sustainable tool for advancing livestock health and productivity, evidence- based validation within Sub-Saharan African contexts is essential prior to widespread application.

Keywords : Rural Livestock Systems, Sustainable Farming Innovations; Cattle Productivity.

References :

1. Abuelo, A., Hernandez, J., Benedito, J. L., & Castillo, C. (2015). The importance of the oxidative status of dairy cattle in the periparturient period: Revisiting antioxidant supplementation. Journal of Animal Physiology and Animal Nutrition, 99, 1003–1016.
2. Angelos, J. A. (2015). Infectious bovine keratoconjunctivitis (pinkeye). Veterinary Clinics of North America: Food Animal Practice, 31(1), 61–79.
3. Ateş, F. M. (2020). Water, salt, hypochlorous acid and infection protection. Bayburt Üniversitesi Fen Bilimleri Dergisi, 3, 154–160.
4. Arthur, T. M., Bosilevac, J. M., Nou, X., Shackelford, S. D., Wheeler, T. L., Kent, M. P., Karoni, D., Pauling, B., Allen, D. M., & Koohmaraie, M. (2004). Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriaceae, and Escherichia coli O157 at various steps in commercial beef processing plants. Journal of Food Protection, 67(4), 658–665.
5. Babur, M., & Karademir, B. (2023). The comparison of ketokonazol and hypochlorous acid (HOCl) applications for the treatment of fungal infections (dermatophytosis). Turkish Journal of Agriculture-Food Science and Technology, 11(4), 791–798.
6. Bajgai, J., Kim, C. S., Rahman, M., Fadriquela, A., Thuy, T., & Lee, K. (2020). Application of new concept disinfectant, Huureka®, on livestock farming.
7. Block, M. S., & Rowan, B. G. (2020). Hypochlorous acid: A review. Journal of Oral and Maxillofacial Surgery, 78(8), 1271–1276.
8. Cavalheiro, L. G., Gené, L. A., Coldebella, A., Kich, J. D., & Ruiz, V. L. A. (2022). Microbiological quality of pig carcasses in a slaughterhouse under risk-based inspection system. Foods, 11(24), 3986.
9. Centurión-Castro, F., Orihuela-Porcayo, J., Aké-López, R. J., Magaña-Monforte, J. G., Montes-Pérez, R. C., & Segura-Correa, J. C. (2013). Effect of body condition score on estrus and ovarian function characteristics of synchronized beef-master cows. Tropical and Subtropical Agroecosystems, 16(2), 193–199.
10. Devant, M., Verdú, M., Medinyà, C., Riera, J., & Marti, S. (2019). PSXII-10 Voluntary water intake of calves and fattening Holstein bulls fed high-concentrate diets. Journal of Animal Science, 97(S3), 410–411. https://doi.org/10.1093/jas/skz258.814
11. Fam, A., Finger, P. T., Tomar, A. S., Garg, G., & Chin, K. J. (2020). Hypochlorous acid antiseptic washout improves patient comfort after intravitreal injection: A patient-reported outcomes study. Indian Journal of Ophthalmology, 68, 2439–2444.
12. Gard, J., Taylor, D., Maloney, R., Schnuelle, M., Duran, S., Moore, P., Justus, W., Walz, P., Stockle, R., Rodning, S., DeGraves, F., van Santen, E., Edmonson, M., & O’Conner, A. M. (2016). Preliminary evaluation of hypochlorous spray for treatment of experimentally induced infectious bovine keratoconjunctivitis. The Bovine Practitioner, 50(2), 180–189
13. Gellynck, X., Messens, W., Halet, D., Grijspeerdt, K., Hartnett, E., & Viaene, J. (2008). Economics of reducing Campylobacter at different levels within the Belgian poultry meat chain. Journal of Food Protection, 71(3), 479–485.
14. Gold, M. H., Andriessen, A., Bhatia, A. C., Bitter Jr, P., Chilukuri, S., Cohen, J. L., & Robb, C. W. (2020). Topical stabilized hypochlorous acid: The future gold standard for wound care and scar management in dermatologic and plastic surgery procedures. Journal of Cosmetic Dermatology, 19, 270–277.
15. Gokce, K. Dogan, D.  Ilhan, FM. Midi A (2023) Giant Hydatid Cyst Originating from Psoas Muscle Extending to the Iliac Bone, Inguinal, and Femoral Canals: A Case Report and Current Literature Review.  DOI: 10.4274/haseki.galenos.2023.9518; Med Bull Haseki;61:379-383
16. Goto, K. (2015). Use of hypochlorous acid solution as a disinfectant in laboratory animal facilities. Annals of Clinical and Medical Microbiology, 1, 1005.
17. Gray, D., Foster, K., Cruz, A., Kane, G., Toomey, M., & Bay, C. (2016). Universal decolonization with hypochlorous solution in a burn intensive care unit in a tertiary care community hospital.  American Journal of Infection Control, 44(9), 1044–1046.

18. Hakim, H., Thammakarn, C., Suguro, A., Ishida, Y., Kawamura, A., Tamura, M., Satoh, K., Tsujimura, M., & Takehara, K. (2015a). Evaluation of sprayed hypochlorous acid solutions for their virucidal activity against avian influenza virus through in vitro experiments. Journal of Veterinary Medical Science, 77(2), 211–215.
19. Hakim, H., Thammakarn, C., Suguro, A., Ishida, Y., Nakajima, K., Kitazawa, M., & Takehara, K. (2015b). Aerosol disinfection capacity of slightly acidic hypochlorous acid water towards Newcastle disease virus in the air: an in vivo experiment. Avian Diseases, 59, 486–491.
20. Hansen, B.G., & Østerås, O. (2019). Farmer welfare and animal welfare-Exploring the relationshipbetween farmer’s occupational well-being and stress, farm expansion and animal welfare. Preventive Veterinary Medicine, 170, 104741.
21. Hao, X., Cao, W., Li, B., Zhang, Q., Wang, C., & Ge, L. (2014). Slightly acidic electrolyzed water for reducing airborne microorganisms in a layer breeding house. Journal of the Air & Waste Management Association, 64(4), 494–500.
22. Hiebert, J. M., & Robson, M. C. (2016). The immediate and delayed post debridement effects on tissue bacterial wound counts of hypochlorous acid versus saline irrigation in chronic wounds. Eplasty, 16, e32.
23. Huang Y, Yen-Con Hung, Shun-Yao Hsu, Yao-Wen Huang, Deng-Fwu Hwang, (2008) Application of electrolyzed water in the food industry, Food Control,Vol 19, Iss 4; Pages 329-345; ISSN 0956-7135, https://doi.org/10.1016/j.foodcont.2007.08.012. (https://www.sciencedirect.com/science/article/pii/S0956713507001697)
24. Joachim, D. (2020). Wound cleansing: Benefits of hypochlorous acid. Journal of Wound Care, 29, S4–S8.
25. Kanclerz, P., Grzybowski, A., & Olszewski, B. (2019). Low efficacy of hypochlorous acid solution compared to povidone-iodine in cataract surgery antisepsis. The Open Ophthalmology Journal, 13, 29–33.
26. Kolářová I, Florent I, Valigurová A. (2022) 10 Parasitic Protists: Diversity of Adaptations to a Parasitic Lifestyle. Microorganisms. (8):1560. doi: 10.3390/microorganisms10081560. PMID: 36013978; PMCID: PMC9414628.
27. Kim, Y-R., & Nam, S-H. (2018). Comparison of the preventive effects of slightly acidic HOCl mouthwash and CHX mouthwash for oral diseases. Biomedical Research, 29, 1718–1723.
28. Kubota, A., Goda, T., Tsuru, T., et al. (2015). Efficacy and safety of strong acid electrolyzed water for peritoneal lavage to prevent surgical site infection in patients with perforated appendicitis. Surgery Today, 45, 876.
29. Llonchi, L., Verdú, M., Martí, S., Medinyà, C., Riera, J., Cucurull, J., & Devant, M. (2023). Drinking water chlorination in dairy beef fattening bulls: water quality, potential hazards, apparent total tract digestibility, and growth performance. International Journal of Animal Bioscience, Animal, 17, 100685.
30. Mapiye, C., Chimonyo, M., Dzama, K., & Marufu, M. C. (2010). Protein Status of Indigenous Nguni and Crossbred Cattle in the Semi-arid Communal Rangelands in South Africa. Asian-Australasian Journal of Animal Sciences, 23(2), 213–225.
31. McParland, S., Dillon, P. G., Flynn, J., Ryan, N., Arkins, S., & Kennedy, A. (2019). Effect of using internal teat sealant with or without antibiotic therapy at dry-off on subsequent somatic cell count and milk production. Journal of Dairy Science, 102(5), 4464–4475.
32. Mwangi, M. and Kariuki, S. (2015) Factors Determining Adoption of New Agricultural Technology by Smallholder Farmers in Developing Countries. Journal of Economics and Sustainable Development, 6, 208-216
33. Naka, A., Yakubo, M., Nakamura, K., & Kurahashi, M. (2020). Effectiveness of slightly acidic electrolyzed water on bacteria reduction: in vitro and spray evaluation. PeerJ, 8, e8593.
34. Natarelli, N., Nong, Y., Maloh, J., & Sivamani, R. (2022). Hypochlorous Acid: Applications in Dermatology. Journal of Integrated Dermatology. .
35. Nickerson, S. C., Kautz, F. M., Ely, L. O., & Ryman, V. E. (2019). Germicidal efficacy testing of Forticept Udder Wash (pre-dip) and Forticept Udder Forte (post-dip) in reducing the new intramammary infection rate and SCC under natural exposure to mastitis pathogens, with additional in vitro testing of germicidal activity. https://api.semanticscholar.org/CorpusID:201677126
36. Odorcic, S., Haas, W., Gilmore, M. S., & Dohlman, C. H. (2015). Fungal Infections Following Boston Type 1 Keratoprosthesis Implantation: Literature Review and In Vitro Antifungal Activity of Hypochlorous Acid. Cornea, 34, 1599–1605.
37. Pelgrift, R. Y., & Friedman, A. J. (2013). Topical Hypochlorous Acid (HOCl) as a Potential Treatment of Pruritus. Current Dermatology Reports, 2(3), 181–190.
38. Pullar, J. M., Winterbourn, C. C., & Vissers, M. C. (1999). Loss of GSH and thiol enzymes in endothelial cells exposed to sublethal concentrations of hypochlorous acid. American Journal of Physiology-Heart and Circulatory Physiology, 277(4), H1505-H1512.
39. Rahman, H., Kim, C.-S., & Lee, K.-J. (2021). Hydrogen-mineral disinfectant water and its application in agriculture and livestock farming. Korean Journal of Agricultural Science, 9(1), 33-33.
40. Rahman, S. M. E., et al. (2016). Electrolyzed water as an antimicrobial agent. Critical Reviews in Food Science and Nutrition, 56(16), 2566–2577.
41. Romanowski, E. G., Yates, K. A., Romanowski, J. E., Mammen, A., Dhaliwal, D. K., Jhanji, V., Shanks, R. M., & Kowalski, R. P. (2020). The Disinfection of Bacterial, Fungal, and Viral Contaminated Contact Lenses and Cases with Hypochlorous Acid. Investigative Ophthalmology & Visual Science, 61, 413–413.

42. Sakarya, S., Gunay, N., Karakulak, M., Ozturk, B., & Ertugrul, B. (2014). Hypochlorous Acid: An Ideal Wound Care Agent With Powerful Microbicidal, Antibiofilm, and Wound Healing Potency. Wounds, 26(12), 342–350.
43. Stroman, D. W., Mintun, K., Epstein, A. B., et al. (2017). Reduction in bacterial load using hypochlorous acid hygiene solution on ocular skin.Clinical Ophthalmology, 11, 707.
44. USDA. (2015) Hypochlorous acid handling
45. United State Development Agency. (2017). National Organic Program Handbook. United States Department of Agriculture.
46. USDA (2018) National Organic Program(NOP);  revision to the national list of allowed and prohibited substances (crops, livestock and handling). a rule by the agricultural mar-keting service (AMS) on 12/27/2018.
47. Vargas-Bello-Pérez, E., Cruz-Morales, S., Dhakal, R., & Hansen, H. H. (2021). Long-term effects of electrochemically activated drinking water on milk yield, milk composition and somatic cell counts in dairy cows: a field study. Journal of Applied Animal Research, 49(1), 304–308.
48. Vargas-Bello-Perez, E., Dhakal, R., Nielsen, M. O., Ahrne, L., & Hansen, H. H. (2020). Short communication: effects of electrochemically activated drinking water on bovine milk production and composition, including chlorate, perchlorate, and fatty acid profile. Journal of Dairy Science, 103(2), 1208–1214.
49. Veasey, S. and Muriana, P. (2016) Evaluation of Electrolytically-Generated Hypochlorous Acid (‘Electrolyzed Water’) for Sanitation of Meat and Meat-Contact Surfaces; Foods
50. Viltrop, A., Niine, T., Tobias, T., Sassu, E. L., Di Bartolo, L., Pavoni, E., Alborali, G. L., Burow, E., & Smith, E. P. (2023). A Review of Slaughter Practices and Their Effectiveness to Control Microbial– esp. Salmonella spp. – Contamination of Pig Carcasses. Journal of Food Protection, 86.
51. Whiteman, M., Chu, S. H., Siau, J. L., Rose, P., Sabapathy, K., Schantz, J. T., et al. (2007). The pro-inflammatory oxidant hypochlorous acid induces Bax-dependent mitochondrial permeabilisation and cell death through AIF-/EndoG-dependent pathways. Cellular Signalling, 19, 705–714.
52. WHO. (2022). Water, sanitation and hygiene: Guidelines and standards. World Health Organization.
53. Williams, J., Rasmussen, E. R., & Lori. (2017). Hypochlorous Acid: Harnessing the Innate Defense Response. Infection Control Tips. https://infectioncontrol.tips/2017/10/06/hypochlorous-innate-response/
54. Winterbourn, C. C., & Kettle, A. J. (2000). Biomarkers of HOCl activity and oxidative stress. Free Radical Biology and Medicine, 29(5), 403–409.
55. Yan, P., Daliri, E. B., & Oh, D.-H. (2021). New Clinical Applications of Electrolyzed Water: A Review.Microorganisms, 9, 136.
56. Young, I., Wilhelm, B. J., Cahill, S., Nakagawa, R., Desmarchelier, P., & Rajić, A. (2016). A rapid systematic review and meta-analysis of the efficacy of slaughter and processing interventions to control nontyphoidal salmonella in beef and pork. Journal of Food Protection, 79(12), 2196–2210.
57. Zastempowska, E., Grajewski, J., & Twaruzek, M. (2017). Food-borne pathogens and contaminants in raw milk—A review. Annals of Animal Science, 16(3), 623–639.