Identification and Antimicrobial Activity of Lactic Acid Bacteria Isolate from Fermented Cassava (Growol) to Bacillus cereus and Morganella morganii


Authors : Henry Dharma Iskandar; Ekawati Purwijantiningsih; Ignatius Pramana Yuda; Benediktus Yudo Leksono

Volume/Issue : Volume 9 - 2024, Issue 1 - January

Google Scholar : http://tinyurl.com/4496pvkc

Scribd : http://tinyurl.com/2eavtcks

DOI : https://doi.org/10.5281/zenodo.10580108

Abstract : Most of the gastrointestinal infections (GI) are dominated by pathogenic bacteria, such as Bacillus cereus and Morganella morganii, which are spread through lack of hygiene in food. Increasing the intestinal microflora could prevent gastrointestinal infections. Intestinal microflora can be increased by consuming foods containing bacteria which have the potency to inhibit the growth of pathogenic microbes in digestive tract such as Lactic Acid Bacteria (LAB). LAB is usually found in fermented foods and growol, Indonesian local spontaneous fermented cassavas, is one of them. This study aims to identify LAB species in growol using molecular techniques and its antimicrobial activity. Identification of LAB isolates and the antimicrobial activity were done using 16S rRNA Gene Sequencing and Well Diffusion Method using Bacillus cereus and Morganella morganii as strain indicator. Results showed 5 LAB isolates can be isolated and had the characteristics of Gram positive, non-motile, and catalase negative. The 16S rRNA Gene Sequencing identified 4 Enterococcus faecium, and 1 Enterococcus durans. Identification of 5 isolates that been sequenced can be seen on accession number (MH793509 – MH793513). Antimicrobial activity of the Cell-Free Supernatants from 5 isolates inhibits Bacillus cereus (d = 2,48 mm) and Morganella morganii (d = 2,68 mm) and can be categorized as weak (0-3 mm).

Keywords : Lactic Acid Bacteria (LAB); Growol; Cell-Free Supernatants (CFS); Enterococcus; Bacillus cereus; Morganella morganii.

Most of the gastrointestinal infections (GI) are dominated by pathogenic bacteria, such as Bacillus cereus and Morganella morganii, which are spread through lack of hygiene in food. Increasing the intestinal microflora could prevent gastrointestinal infections. Intestinal microflora can be increased by consuming foods containing bacteria which have the potency to inhibit the growth of pathogenic microbes in digestive tract such as Lactic Acid Bacteria (LAB). LAB is usually found in fermented foods and growol, Indonesian local spontaneous fermented cassavas, is one of them. This study aims to identify LAB species in growol using molecular techniques and its antimicrobial activity. Identification of LAB isolates and the antimicrobial activity were done using 16S rRNA Gene Sequencing and Well Diffusion Method using Bacillus cereus and Morganella morganii as strain indicator. Results showed 5 LAB isolates can be isolated and had the characteristics of Gram positive, non-motile, and catalase negative. The 16S rRNA Gene Sequencing identified 4 Enterococcus faecium, and 1 Enterococcus durans. Identification of 5 isolates that been sequenced can be seen on accession number (MH793509 – MH793513). Antimicrobial activity of the Cell-Free Supernatants from 5 isolates inhibits Bacillus cereus (d = 2,48 mm) and Morganella morganii (d = 2,68 mm) and can be categorized as weak (0-3 mm).

Keywords : Lactic Acid Bacteria (LAB); Growol; Cell-Free Supernatants (CFS); Enterococcus; Bacillus cereus; Morganella morganii.

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