Authors :
Henry Dharma Iskandar; Ekawati Purwijantiningsih; Ignatius Pramana Yuda; Benediktus Yudo Leksono
Volume/Issue :
Volume 9 - 2024, Issue 1 - January
Google Scholar :
http://tinyurl.com/4496pvkc
Scribd :
http://tinyurl.com/2eavtcks
DOI :
https://doi.org/10.5281/zenodo.10580108
Abstract :
Most of the gastrointestinal infections (GI) are
dominated by pathogenic bacteria, such as Bacillus cereus
and Morganella morganii, which are spread through lack
of hygiene in food. Increasing the intestinal microflora
could prevent gastrointestinal infections. Intestinal
microflora can be increased by consuming foods
containing bacteria which have the potency to inhibit the
growth of pathogenic microbes in digestive tract such as
Lactic Acid Bacteria (LAB). LAB is usually found in
fermented foods and growol, Indonesian local
spontaneous fermented cassavas, is one of them. This
study aims to identify LAB species in growol using
molecular techniques and its antimicrobial activity.
Identification of LAB isolates and the antimicrobial
activity were done using 16S rRNA Gene Sequencing and
Well Diffusion Method using Bacillus cereus and
Morganella morganii as strain indicator. Results showed
5 LAB isolates can be isolated and had the characteristics
of Gram positive, non-motile, and catalase negative. The
16S rRNA Gene Sequencing identified 4 Enterococcus
faecium, and 1 Enterococcus durans. Identification of 5
isolates that been sequenced can be seen on accession
number (MH793509 – MH793513). Antimicrobial activity
of the Cell-Free Supernatants from 5 isolates inhibits
Bacillus cereus (d = 2,48 mm) and Morganella morganii
(d = 2,68 mm) and can be categorized as weak (0-3 mm).
Keywords :
Lactic Acid Bacteria (LAB); Growol; Cell-Free Supernatants (CFS); Enterococcus; Bacillus cereus; Morganella morganii.
Most of the gastrointestinal infections (GI) are
dominated by pathogenic bacteria, such as Bacillus cereus
and Morganella morganii, which are spread through lack
of hygiene in food. Increasing the intestinal microflora
could prevent gastrointestinal infections. Intestinal
microflora can be increased by consuming foods
containing bacteria which have the potency to inhibit the
growth of pathogenic microbes in digestive tract such as
Lactic Acid Bacteria (LAB). LAB is usually found in
fermented foods and growol, Indonesian local
spontaneous fermented cassavas, is one of them. This
study aims to identify LAB species in growol using
molecular techniques and its antimicrobial activity.
Identification of LAB isolates and the antimicrobial
activity were done using 16S rRNA Gene Sequencing and
Well Diffusion Method using Bacillus cereus and
Morganella morganii as strain indicator. Results showed
5 LAB isolates can be isolated and had the characteristics
of Gram positive, non-motile, and catalase negative. The
16S rRNA Gene Sequencing identified 4 Enterococcus
faecium, and 1 Enterococcus durans. Identification of 5
isolates that been sequenced can be seen on accession
number (MH793509 – MH793513). Antimicrobial activity
of the Cell-Free Supernatants from 5 isolates inhibits
Bacillus cereus (d = 2,48 mm) and Morganella morganii
(d = 2,68 mm) and can be categorized as weak (0-3 mm).
Keywords :
Lactic Acid Bacteria (LAB); Growol; Cell-Free Supernatants (CFS); Enterococcus; Bacillus cereus; Morganella morganii.
