Authors : Azhari; Ismail; Rastina

Volume/Issue : Volume 9 - 2024, Issue 5 - May

Google Scholar : https://tinyurl.com/3ya3bmkm

Scribd : https://tinyurl.com/49fdzux3

DOI : https://doi.org/10.38124/ijisrt/IJISRT24MAY2495

Abstract : The use of feed ingredients has not been addressed until now, in the sense that competition between food and feed is still continuing, especially protein source feed, thus causing a dilemma for farmers.Therefore, in connection with the steps that will be taken in efforts to health livestock and fish farming that will be carried out, efforts are needed in preventing bacterial-based diseases. The purpose of this study was to determine the diversity of all bacteria, both pathogenic and non-pathogenic bacteria found in the magot digestive system using 16S rRNA gene analysis. The results of this study will be a scientific reference for the development of maggot asasafe, effective, and efficient alternative to animal feed.Based on the results of the studies obtained, it can be concluded that the bacteria contained in the digestion of maggots show a diversity of pathogenic and non-pathogenic bacteria, with the form of bacil and cocobacil. Inaddition, DNA extraction and ba cterial identification have also been carried out using the PCR method. The results of the sequencing of the seven bacterial isolates of magot digestive origin showed gooddendograms and nucleotide sequences, so the next stage was bioinformatics analysis and phylogenetic construction, to identify species and their kinship relationships between species.

Keywords : Maggots, PCR, Larvae, Insecta, Animal Feed.

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