Background: Male reproductive health is a pivotal component of overall well-being, and its susceptibility to environmental factors, including plant extracts, necessitates thorough investigation. Datura stramonium, commonly known as jimsonweed, is renowned for its medicinal properties, yet its influence on male reproductive parameters remains inadequately explored.This study scrutinized the impact of Datura stramonium extract (DSE) on male reproductive parameters in adult male Wistar rats. DSE was derived from the plant's seeds via methanol extraction. Forty- nine rats were distributed into seven groups, receiving 50, 100 and 200 mg/kg DSE doses for 56 days, with some groups undergoing withdrawal for an additional 28 days.Result: Proximate analysis unveiled DSE's composition. The investigation encompassed alterations in body weight, epididymis weight, hormone levels (FSH, LH, PRL, PRG, estrogen, testosterone), and semen quality and morphology. Furthermore, histological evaluations of the epididymis were conducted.Proximate analysis delineated DSE's composition. Administration of diverse DSE doses significantly perturbed body weight, epididymis weight, and hormonal levels, affecting Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), testosterone, and others. Semen analysis exposed changes in sperm count, motility, and morphology. Remarkably, some effects were ameliorated upon DSE withdrawal. Histological examinations authenticated structural modifications in the epididymides, paralleling the functional alterations observed. In conclusion, Datura stramonium seed extract (DSE) considerably impaired male reproductive parameters, yet withdrawal exhibited partial recovery.Subsequent research is imperative for comprehending the enduring repercussions on reproductive health.

Keywords : Epididymis, Datura stramonium Extract, Hormonal Imbalance, Semen Quality, Withdrawal Effects