This study at PT Manunggal Jaya Makmur intends to examine the effects of organizational culture and work environment on workers' occupational safety and health using work motivation as a mediating variable. All employees make up the study's population, which included a sample size of 50 people. The Structural Equation Model-Partial Least Square (SEM-PLS) data analysis technique is used. Based on the findings of the study and the discussion, it can be said that organizational culture affects occupational safety and health in a positive and significant way. This indicates that increasing organizational culture's level of self- awareness will improve occupational safety and health. A strong company culture will boost employee motivation since it has a favorable, significant impact on job motivation. The working environment has a positive and significant impact on occupational safety and health, therefore working conditions in terms of employee interactions, specifically employee cooperation, have an effect on enhancing occupational safety and health. A good work environment will influence employee work motivation since the work environment has a positive and significant impact on it. Workplace safety and health are dramatically improved by work motivation. Workplace safety and health will both rise dramatically with a strong organizational culture. Workplace harmony will greatly boost safety and health at work as well as employee motivation.

Keywords : Occupational Safety and Health (OSH), Work Motivation, Organizational Culture.