The major objectives of the study has been made to examine the role of physical factors like strength, speed, endurance, agility and flexibility on the performance of the Kho-kho players. It offers great movements in the body which are specifically measured on motor test like speed, endurance, strength, agility and flexibility by following available standard norms in the field of sports. A large sample was selected randomly on the whole physical factors and physiological factors. They were administered with tests of resting heart rate, peak heart rate, aerobic capacity, RBC and Hemoglobin. After scoring the required sample (100) as per sample design was selected thus an equal number of players were selected on the factor on whom the motor tests were conducted as per norms. The scores were subjected to statistical analysis.