Authors : Aadil Ansari; Abhishek Nagar; Rajkumari Thagele

Volume/Issue : Volume 11 - 2026, Issue 6 - June

Google Scholar : https://tinyurl.com/mt7ej5zr

Scribd : https://tinyurl.com/mu6d78sn

DOI : https://doi.org/10.38124/ijisrt/26jun1163

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Abstract : In vitro antioxidant activity was evaluated using DPPH and hydrogen peroxide scavenging assays, where the extract demonstrated dosedependent radical scavenging activity comparable to ascorbic acid. Further, cytoprotective effects were assessed using H9c2 cardiomyocyte cell lines. The extract significantly improved cell viability in oxidative stress-induced conditions (H₂O₂ exposure) as evidenced by MTT assay results. Additionally, intracellular ROS levels were markedly reduced in treated groups, confirming its antioxidant efficacy. Statistical analysis indicated significant reduction in ROS levels at higher concentrations (p < 0.001), highlighting the potent cardioprotective potential of the extract. Overall, the findings suggest that Myristica fragrans possesses substantial antioxidant properties that may contribute to its protective effects against oxidative stress-induced cardiac damage.

Keywords : Myristica fragrans, Cardioprotective Activity, Oxidative Stress, Reactive Oxygen Species (ROS), Antioxidants, Flavonoids, Phenolic Compounds, DPPH Assay, H9c2 Cell Line.

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