Orchidaceae is the largest family comes at
second place in beautiful as well as sunshine flowering
plants. Orchids are used as sources of medicine. Orchid
is one of the major flowering plants with high demand in
international market due to multifaced powerful
aphrodisiac qualities. Orchid’s are propagated by
standard conventional methods which is very slow
process. Vegetative propagation occurs through division
of clumps, rhizomes, cutting and off shoots separation.
Seed germination requires other supplementary
techniques in natural conditions. As compare to natural
propagation methods, plant tissue culture technique
provides axenic in vitro propagation of orchids in large
scale with quality plantlets in short period of time.
Numerous factors are important for successful micro
propagation such as explant collection, pH and
temperature, size of explant, environmental conditions,
culture medium, PGR, etc. Sequential study of
experiments helpful for understanding biological,
physical and physiochemical aspect that govern in vitro
morphogenesis of orchids. Plant tissue culture also aim’s
in soma clonal variations of orchids for creations of
Keywords : In Vitro, Orchid Species, PGR, Problems and Solutions.