In Vitro Root Induction and Acclimatization of Asian Pear (Pyrus betulifolia) in Field


Authors : Nyo Zin Hlaing, Jiang Shuling

Volume/Issue : Volume 4 - 2019, Issue 7 - July

Google Scholar : https://goo.gl/DF9R4u

Scribd : https://bit.ly/35q7FhZ

Abstract : Explants of P.betulifolia were tested for micropropagation and rooting in vitro and acclimatization in vivo. In multiplication test, BA 0.5 mg/l combination was the best proliferation rate. Rooted shoots, root length and rooting percent were resulted the best in this data as 3.35, 2.33 cm and 67% respectively in 1⁄2 MS + 0.3 mg /l with dipping 10 second in 2 mg/l IBA (50% EtOH). But 1⁄2MS + 0.2 mg/l IBA with dipping 15s in the same IBA concentration provided the best number of roots (4.30). According to the rooting data, 1⁄2MS + 0.2 and 0.3 mg/l IBA were given roots but they were not good results as dipping method. Finally, the healthy survived 37.10% pear from in vitro rooted plants was grown in fields successfully.

Keywords : P.betulifolia, Acclimatization, 1⁄2MS, BA, IBA, EtOH. Abbreviation: MS - Murashige and Skoog BA - 6-Benzyladenine IBA -Indole-3-butyric acid IAA- Indole-3-acetic acid Zt -Zeatin GA3- Gibberellic acid

Explants of P.betulifolia were tested for micropropagation and rooting in vitro and acclimatization in vivo. In multiplication test, BA 0.5 mg/l combination was the best proliferation rate. Rooted shoots, root length and rooting percent were resulted the best in this data as 3.35, 2.33 cm and 67% respectively in 1⁄2 MS + 0.3 mg /l with dipping 10 second in 2 mg/l IBA (50% EtOH). But 1⁄2MS + 0.2 mg/l IBA with dipping 15s in the same IBA concentration provided the best number of roots (4.30). According to the rooting data, 1⁄2MS + 0.2 and 0.3 mg/l IBA were given roots but they were not good results as dipping method. Finally, the healthy survived 37.10% pear from in vitro rooted plants was grown in fields successfully.

Keywords : P.betulifolia, Acclimatization, 1⁄2MS, BA, IBA, EtOH. Abbreviation: MS - Murashige and Skoog BA - 6-Benzyladenine IBA -Indole-3-butyric acid IAA- Indole-3-acetic acid Zt -Zeatin GA3- Gibberellic acid

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