The bacterial growth associated with earphones used among students of Saveetha Medical College and Hospital, Chennai was investigated. A total 60 earphone users were randomly selected. Swabs were taken from the ear canal and samples were analyzed using standard microbiological methods. The bacteria isolated from the ear swabs include; Pseudomonas and Staphylococcus aureus. The distribution of bacterial isolates includes; Pseudomonas (21.5%), Staphylococcus (15.1%), and no organism isolated (63.4%). The earphone users were categorized under four categories depending upon the duration of daily usage of earphones; 0-15 minutes (Group A), 30-45 minutes (Group B), 1-2 hours (Group C), more than 2 hours (Group D). By comparing the occurrence values of the isolates between the four categories, of earphone users, the study concluded that bacterial growth does increase with frequent and continuous use of earphones. The significantly higher occurrence values of isolates in Group D when compared to the other groups are indicative of a person relationship between bacterial frequency and duration of use of earphones. Therefore, regular cleaning of earphones with disinfectants before and after each usage is recommended to help reduce the bacterial load of earphones and to help prevent the incidence of otitis media and other ear infections amongst the earphone users (1).