Influence of Microbial Biofertilizers on Germination of Millet Varieties and Millet use in Biocontrol

Authors : Danladi, G. H; Muhammad, S; Aliero, A. A; Isah, A. D; Gupta, A

Volume/Issue : Volume 6 - 2021, Issue 12 - December

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Influence on germination of Dansalka and Bahaushe millet (Pennisetum glaucum L. Br.) varieties by biofertilizers produced from Azotobacter vinelandi and Rhizobium phaseoli along with bacterial control abilities of millet grains extract were studied using laboratory facilities at Usmanu Danfodiyo University, Sokoto and Center for Microbiology and Biotechnology, Bhopal. Biofertilizers were produced from both microorganisms using upstream and downstream fermentation method, with Ashby and yeast extract mannitol broths as media. Three working concentrations of 10, 15 and 20mg/ml were obtained from each biofertilizer. Standard methods were adopted for the germination studies, treatments were replicated three times. Millet extracts of 60:40 water and alcohol, were tested for in-vitro bacterial control by plate disc method. Second day after sowing revealed no germination from controls, but biofertilizer treatments recorded highest germination rate from 20ml/l treatments resulting in 63% for Dansalka in Azotobacter, 57% for Dansalka and Bahaushe in Rhizobium and 53% for Bahaushe in Rhizobium. The 15ml/l treatments followed with 37% Bahaushe in Rhizobium and 33% for other millets in both fertilizers. Full (100%) germination was in 20ml/l from 3rd day and by 4th day, all 20ml/l and 15ml/l treated millets fully germinated. Dansalka in 10ml/l Azotobacter germinated 100% on day 4, while the control had 87% as final germination. Highest extract inhibition was 15.33mm against A. vinelandi followed by 14.33mm against R. phaseoli by 400mg/l. The lowest inhibition was 3.67mm on Xanthomonas axonopodis and X. campestris by 300mg/l. The 200mg/l showed no inhibition. Both fertilizers positively influenced millet germination while extract at highest concentration had mild inhibition of the microorganisms. Therefore, Azotobacter and Rhizobium biofertilizers are recommended especially for organic millet cultivation

Keywords : Millet Microorganisms Biofertilizers Germination Inhibition


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