Authors :
Trimurni Abidin; Fitri Yunita Batubara; Harry Agusnar; Basri A. Gani
Volume/Issue :
Volume 7 - 2022, Issue 4 - April
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3H7LVe1
DOI :
https://doi.org/10.5281/zenodo.6626261
Abstract :
Streptococcus mutans are oral bacteria that
have the most role in developing dental caries. S.
mutanscan form colonies and biofilms through adhesion
mechanisms on the tooth surface. The use of chitosan as
an antimicrobial material has been widely developed in
dentistry, especially on the nanoscale, because
nanomaterials can spread quickly throughout the
biofilm structure. The use of hydroxyapatite (HAP) in
conservative dentistry has been developed to protect
teeth from S. mutans. This research aims to see the
ability of nano chitosan hydroxyapatite (KNHP), if
combined in gel form, does it prevent S. mutansfrom
forming biofilms on the enamel surface. The research
material was S. mutansserotype c and saliva collected
from a volunteer. The biofilm mass profile was
determined on the surfaces of teeth implanted on acrylic
resin and incubated for 24, 48, and 72 hours, then
observed under a counter colony lens. Determination of
S. mutans CFU after interacting with nano chitosan
hydroxyapatite for 24, 48, and 72 was then examined
with spectrophotometry at a wavelength of 610 nm OD
value of 0.08-0.1, which is equivalent to 0.5 McFarlan
(1.5x 108). Then the determination of the interaction
power of KNHP and HAP with S. mutanswas done by
UV / VIS with a wavelength of 250-600 nm. The results
showed that the mass profile of biofilms at 24 hours still
showed colony growth after being given KNHP and
HAP, whereas, at incubation times at 48 and 72 hours,
there was no S. mutans growth. The interaction between
KNHP and S. mutans with UV-VIS at 24, 48, and 72
hours incubation time has higher interaction power
than HAP.
Keywords :
Biofilm, Nano chitosan hydroxyapatite, Streptococcus mutans
Streptococcus mutans are oral bacteria that
have the most role in developing dental caries. S.
mutanscan form colonies and biofilms through adhesion
mechanisms on the tooth surface. The use of chitosan as
an antimicrobial material has been widely developed in
dentistry, especially on the nanoscale, because
nanomaterials can spread quickly throughout the
biofilm structure. The use of hydroxyapatite (HAP) in
conservative dentistry has been developed to protect
teeth from S. mutans. This research aims to see the
ability of nano chitosan hydroxyapatite (KNHP), if
combined in gel form, does it prevent S. mutansfrom
forming biofilms on the enamel surface. The research
material was S. mutansserotype c and saliva collected
from a volunteer. The biofilm mass profile was
determined on the surfaces of teeth implanted on acrylic
resin and incubated for 24, 48, and 72 hours, then
observed under a counter colony lens. Determination of
S. mutans CFU after interacting with nano chitosan
hydroxyapatite for 24, 48, and 72 was then examined
with spectrophotometry at a wavelength of 610 nm OD
value of 0.08-0.1, which is equivalent to 0.5 McFarlan
(1.5x 108). Then the determination of the interaction
power of KNHP and HAP with S. mutanswas done by
UV / VIS with a wavelength of 250-600 nm. The results
showed that the mass profile of biofilms at 24 hours still
showed colony growth after being given KNHP and
HAP, whereas, at incubation times at 48 and 72 hours,
there was no S. mutans growth. The interaction between
KNHP and S. mutans with UV-VIS at 24, 48, and 72
hours incubation time has higher interaction power
than HAP.
Keywords :
Biofilm, Nano chitosan hydroxyapatite, Streptococcus mutans
