In this present research, soil samples were collected from three different places from Magway University, during January to July 2018. Ten different soil fungi were isolated from three different soil samples. Isolation of soil fungi were undertaken by the serial dilution method. A total of 10 fungal cultures were isolated from the collected soil samples. Antimicrobial activities of isolated fungal strains were evaluated by the agar well diffusion assay with five test organisms. There are Bacillus subtilis, Escherichia coli, Pseudomonas fluorescences, Agrobacterium tumefaciens and Staphylococcus aureus. These isolated fungal strains were tested by 4 days culture within fermentation period. Clear zones (inhibitory zones) surrounding the agar well indicate the presence of bioactive metabolites which inhibit the growth of test organisms. Four strains showed the antimicrobial activity on Escherichia coli. Among them Bacillus subtilis fungal strains showed that the maximum antimicrobial activities (30.33 mm) and Pseudomonas fluorescences showed a few antimicrobial activity ( 9.77 mm). According to the present studied that soil fungi are ubiquitous and different morphologies among different soil area and this soil fungi were possess with potential antimicrobial activities.

Keywords : Soil Fungi, Antimicrobial Activity.