Authors :
Malavika S Pradeep; Smitha Mathews; Aleesha Prince; A Salma
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/42nqEHs
DOI :
https://doi.org/10.5281/zenodo.7758396
Abstract :
The enzyme protease hydrolyses protein into
peptides and amino acids. One of the major sources of
protease producing bacterial strains is soil. A total of 30
soil samples were collected aseptically from
Changanacherry, and brought to the laboratory. The
samples were serially diluted and isolated using Nutrient
Agar plates. Skim Agar media was used for the
enumeration of protease producing microorganisms. The
plates were incubated at 370C for 24-48 hours. The pure
culture of 8 colonies exhibiting highest proteolytic
activity was obtained by identifying the clear zones.
After analysing the optimal growth conditions and
antagonistic activities within the strains, 2 isolates were
subjected to antibiotic sensitivity test towards twelve
different antibiotics, microbiological characteristics,
biochemical analysis and extracellular protease enzyme
production. Among the 2 isolates, the one which
exhibited highest protease production was identified as
Bacillus thuringiensis using molecular methods. The
results have increased the scope of finding industrially
important bacteria from soil which can be used as
monoculture or consortium for the degradation of waste
on a large scale.
Keywords :
Soil, Bacterial Strains, Antagonism, Extracellular Protease.
The enzyme protease hydrolyses protein into
peptides and amino acids. One of the major sources of
protease producing bacterial strains is soil. A total of 30
soil samples were collected aseptically from
Changanacherry, and brought to the laboratory. The
samples were serially diluted and isolated using Nutrient
Agar plates. Skim Agar media was used for the
enumeration of protease producing microorganisms. The
plates were incubated at 370C for 24-48 hours. The pure
culture of 8 colonies exhibiting highest proteolytic
activity was obtained by identifying the clear zones.
After analysing the optimal growth conditions and
antagonistic activities within the strains, 2 isolates were
subjected to antibiotic sensitivity test towards twelve
different antibiotics, microbiological characteristics,
biochemical analysis and extracellular protease enzyme
production. Among the 2 isolates, the one which
exhibited highest protease production was identified as
Bacillus thuringiensis using molecular methods. The
results have increased the scope of finding industrially
important bacteria from soil which can be used as
monoculture or consortium for the degradation of waste
on a large scale.
Keywords :
Soil, Bacterial Strains, Antagonism, Extracellular Protease.
