This article describes about the isolation and production of biosurfactants from marine actinomycetes. Few microbes produced biosurfactants has certain chemical properties with high molecular weights which was obtained from living surface of microbial cells exhibited as amphiphiles. Consists of hydrophilic and hydrophobic moieties reduces surface tension. They were used in pharmaceutical applications. TREHALIPIDS, SOPHOROLIPIDS, RHAMNOLIPIDS, PHOSPHOLIPIDS; are the complex compounds present in the biosurfactants and degradation was also studied in In-vitro, produced from the marine organisms of actinomycetes shows antibiotic and probiotic activity. Actinomycetes are a GRAM POSTIVE BACTERIA, comes under the prokaryotic organisms and exhibited as single protein which is a marine sponge collected from south east region for isolation washed with sea water to remove any bacteria to form homogenate, incubated at 400c. colonies, isolated, and by keeping the temperature desired for 24 hours. marine actinomycetes were identified by using their Bio-surfactant properties were investigated using statistical data, and screening methods for specific operations were developed.

Keywords : Amphiphiles, Trehalipids, Sophorolipids, Rhamnolipids, Phospholipids, Gram Positive Bacteria.