Authors :
Amrutha Philip; Smitha Mathews; Dona Thomas; Shradha Sanil
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/408e0KO
DOI :
https://doi.org/10.5281/zenodo.8296656
Abstract :
- The enzyme Amylase catalyses the hydrolysis
of starch into oligosaccharides and sugars. Bacteria
isolated from the soil are the most common sources of
amylase. In this study, soil samples (n=30) collected
from different sites of Changanacherry were taken for
screening amylase-producing bacteria. The samples
were serially diluted and representative bacterial flora
was isolated by plating on the nutrient agar media. The
amylase production of these isolates was assessed using
starch agar. Thirteen isolates were selected for further
investigations. The growth conditions of these isolates
were optimized and they were analysed for antagonistic
activity. Two strains were selected for assessing
antibacterial resistance patterns by the well diffusion
method and to check the antibiotic sensitivity against 12
antibiotics using the disk diffusion method. The
microbiological and biochemical characteristics of these
strains were also determined. They were tested for
extracellular amylase activity at elevated temperatures
using the DNS assay method. The strain showing
maximum amylase activity (2.395 units/ml) was
identified as Alcaligenes faecalis using 16S rDNA-based
method. The selected thermophilic strains can be
employed to degrade starchy waste materials in
monoculture or in a consortium, as well as for the
industrial production of amylase. The study highlights
that there is an increased scope for identifying
industrially important bacteria from soil
Keywords :
Soil, Bacterial strain, Antagonism, Extracellular amylase, Isolates.
- The enzyme Amylase catalyses the hydrolysis
of starch into oligosaccharides and sugars. Bacteria
isolated from the soil are the most common sources of
amylase. In this study, soil samples (n=30) collected
from different sites of Changanacherry were taken for
screening amylase-producing bacteria. The samples
were serially diluted and representative bacterial flora
was isolated by plating on the nutrient agar media. The
amylase production of these isolates was assessed using
starch agar. Thirteen isolates were selected for further
investigations. The growth conditions of these isolates
were optimized and they were analysed for antagonistic
activity. Two strains were selected for assessing
antibacterial resistance patterns by the well diffusion
method and to check the antibiotic sensitivity against 12
antibiotics using the disk diffusion method. The
microbiological and biochemical characteristics of these
strains were also determined. They were tested for
extracellular amylase activity at elevated temperatures
using the DNS assay method. The strain showing
maximum amylase activity (2.395 units/ml) was
identified as Alcaligenes faecalis using 16S rDNA-based
method. The selected thermophilic strains can be
employed to degrade starchy waste materials in
monoculture or in a consortium, as well as for the
industrial production of amylase. The study highlights
that there is an increased scope for identifying
industrially important bacteria from soil
Keywords :
Soil, Bacterial strain, Antagonism, Extracellular amylase, Isolates.