Authors : Tyokusa, A. G.; Danke, I. C.

Volume/Issue : Volume 9 - 2024, Issue 4 - April

Google Scholar : https://tinyurl.com/3aea64u9

Scribd : https://tinyurl.com/3f43earr

DOI : https://doi.org/10.38124/ijisrt/IJISRT24APR399

Abstract : In this study, lactic acid bacteria (LAB) were isolated from fresh milk samples from cow, sheep, and goat using MRS agar as a growth medium. Lactobacillusplantarum, Lactobacillusacidophilus, Lactobacillusfermentum, Enterococcusgallinarum, and Enterococcusfaecium were the species of LAB that were isolated and identified using the Vitek 2 compact system. While Lactobacillus fermentum, Enterococcus faecium, and Lactobacillus plantarum were isolated from goat milk, Lactobacillus acidophilus, Lactobacillus gallinarum, and Lactobacillus plantarum were isolated from cow milk.However, from sheep milk, only Lactobacillus plantarum and Lactobacillus acidophilus were isolated. All the isolates were subjected to amylase production test using modified MRS media and the results showed that Lactobacillus plantarum, Lactobacillusacidophilus and Lactobacillusfermentum produced amylase while Enterococcus gallinarum and Enterococcus faecium did not produce amylase.

Keywords : Milk, LAB, Vitek 2,Amylase and Modified MRS.