Isolation Pigment Producing Microorganism and Pigment Characterization


Authors : Santosh B Patil; Suprita Puttaraj Nalatawad; Savita S Desai; Rajashree Hanagawadimath

Volume/Issue : Volume 10 - 2025, Issue 7 - July


Google Scholar : https://tinyurl.com/awat76yj

Scribd : https://tinyurl.com/ypkt85s4

DOI : https://doi.org/10.38124/ijisrt/25jul275

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Abstract : This research focuses on the isolation and characterization of pigment-producing microorganisms from soil samples collected across different regions of Karnataka. As concerns grow over the environmental and health impacts of synthetic dyes, microbial pigments offer a sustainable alternative with broad applications in food, textiles, cosmetics, and pharmaceuticals. Our study aims to isolate diverse pigment-producing microbes using serial dilution techniques and selective media, followed by comprehensive characterization of the extracted pigments. The pigments will be analyzed using multiple analytical techniques including UV-Visible spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, Thin Layer Chromatography (TLC), and to determine their structural and functional properties. Additionally, we will evaluate the practical application of these pigments in the textile industry by dyeing silk fabrics and assessing color fastness properties according to international standards. This research not only contributes to the growing field of natural colorants but also promotes sustainable practices by potentially utilizing agricultural and industrial by-products in pigment production. The findings of this study may provide valuable insights into novel pigment-producing microorganisms and their potential industrial applications, advancing efforts toward more environmentally friendly and health-conscious coloring alternatives while supporting local economies through sus- tainable biotechnology.

Keywords : Pigment, Bacteria, Isolation, Characterization.

References :

  1. Kim, H., et al. (2020). Taxonomic characterization of Chryseobacterium species from agricultural soil in South Korea. Journal of Microbial Ecology.
  2. Bernardet, J.-F., et al. (2015). Diagnostic protocols for flexirubin detection in Chryseobacterium species. Systematic and Applied Microbiology.
  3. Herzog, P., et al. (2018). Antimicrobial properties of flexirubin-type pigments from soil Chryseobacterium species. Applied Microbiology and Biotechnology.
  4. Chen, L., & Wang, Y. (2019). Optimization of flexirubin pigment production by soil-derived Chryseobacterium species. Journal of Industrial Microbiology & Biotechnology.
  5. Lee, J., et al. (2021). Antioxidant properties of pigments from Chryseobacterium species isolated from soil. Food Chemistry.
  6. Park, S., et al. (2020). Structural characterization of flexirubin pigments in Chryseobacterium. Analytical and Bioanalytical Chemistry.
  7. Zhang, H., et al. (2021). Chitinolytic activity and pigment production in soil-dwelling Chryseobacterium species. Biocontrol Science and Technology.
  8. Singh, A., & Kumar, R. (2022). Genomic insights into flexirubin biosynthesis and stress adaptation in Chryseobacterium. Frontiers in Microbiology.
  9. Rodriguez-Concepcion, M., et al. (2020). Carotenoid components of Chryseobacterium pigments and their ecological implications. Environmental Microbiology.
  10. Müller, C., & Schmidt, F. (2021). Biodegradation of pollutants by pigment-producing Chryseobacterium from soil. Journal of Hazardous Materials.
  11. Wang, Q., et al. (2022). Anti-inflammatory properties of flexirubin pigments in Chryseobacterium species. Phytotherapy Research.
  12. Yamamoto, N., et al. (2021). Textile dyeing with bacterial pigments: Application of Chryseobacterium flexirubins. Dyes and Pigments.

This research focuses on the isolation and characterization of pigment-producing microorganisms from soil samples collected across different regions of Karnataka. As concerns grow over the environmental and health impacts of synthetic dyes, microbial pigments offer a sustainable alternative with broad applications in food, textiles, cosmetics, and pharmaceuticals. Our study aims to isolate diverse pigment-producing microbes using serial dilution techniques and selective media, followed by comprehensive characterization of the extracted pigments. The pigments will be analyzed using multiple analytical techniques including UV-Visible spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, Thin Layer Chromatography (TLC), and to determine their structural and functional properties. Additionally, we will evaluate the practical application of these pigments in the textile industry by dyeing silk fabrics and assessing color fastness properties according to international standards. This research not only contributes to the growing field of natural colorants but also promotes sustainable practices by potentially utilizing agricultural and industrial by-products in pigment production. The findings of this study may provide valuable insights into novel pigment-producing microorganisms and their potential industrial applications, advancing efforts toward more environmentally friendly and health-conscious coloring alternatives while supporting local economies through sus- tainable biotechnology.

Keywords : Pigment, Bacteria, Isolation, Characterization.

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Paper Submission Last Date
31 - December - 2025

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