Authors : Polycarp Ikechukwu Nwabuokei; Doris Onwenna Ngozi; Onyekachukwu Andrew Oginenwa

Volume/Issue : Volume 11 - 2026, Issue 6 - June

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Google Scholar : https://tinyurl.com/5xdhjwyk

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DOI : https://doi.org/10.38124/ijisrt/26jun982

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Abstract : Process parameter optimization showed that ethanol yield was favored at lower temperatures, while elevated temperature and alkaline pH conditions were inhibitory to ethanol production. The optimal hydrolysis conditions were determined to be a temperature of 35 °C, a pH of 5.5, and a hydrolysis period of 5.7 days. Kinetic analysis of the hydrolysis process demonstrated conformity with the Michaelis–Menten model, yielding a maximum reaction rate (Vmax) of 23.81 g·dm⁻³·day⁻¹, a Michaelis–Menten constant (Km) of 20.56 g·dm⁻³, and a coefficient of determination (R²) of 0.99, indicating an excellent fit of the model to the experimental data.

Keywords : Michealis-Menten, Lignocellulosic, Enzymatic Hydrolysis.

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