Pepper Cili Kulai of the variety chilli pepper belonging to family Solanacae is an important vegetable and spice crop grown in Malaysia and produced and consumed as fresh or processed in worldwide. The production is chilli yield products constrained by various plant pathogens causing diseases on chilli seeds, seedling systems and plants including pepper Cili Kulai variety. The main diseases that lead to wilting in chilli are Fusarium wilt and caused huge losses in final products and damage to seedling systems, plant systems in agriculture. In this study, cells and cells free supernatants of the five strains of lactic acid bacterial were used as bio-control agent and energy sources to enhance the seedling systems of Cili Kulai seed. In vitro assay of beneficial microbial supernatants and cells of lactic acid bacteria strains were showed inhibitory activity against fungi Fusarium species and better improvements were noticed that on seedling systems of Cili Kulai seeds in 160 eyes plastic tray filled with soil medium. After two week incubation seeds of Cili Kulai with cells and cells free supernatants of Lactic acid bacteria especially, P. pentosaceus1-MSS, Lb. plantarum1FCF, Lb. acidophilus ATCC314, Lb. plantarum ATCC8014 and Lb. plantarum1-MSS; significantly, were observed that to suppress Fusarium species patho-genicity. The positive impacts were noticed that on seedling systems of Cili Kulai seeds enhancements on seedling systems such as height, shoots and roots length. The negative effect were noticed that on height, shoots and roots elongation when Cili Kulai seeds were survived artificially infected with Fusarium species namely, Fusarium proliferatum-LR, Fusarium sp. fus 124-FC, Fusarium sp. CID124-CS and Fusarium oxysporum KAML01-CL. The better elongation and development on seedling systems were noticed that which Pepper Cili Kulai seeds were survived in presence of cells or cells free supernatants of Lactic acid bacteria either Pepper Cili Kulai seeds and soil were infected with pathogenic Fusarium species. The cells and cells free supernatants of all LAB strains were showed as good energy sources on seedling systems of Cili Kulai and to protect from pathogenic fungi Fusarium species in soil medium.

Keywords : Lactic Acid Bacteria; Cells and supernatants; Fusarium Species; Pepper Cili Kulai; Seedling Systems and Inhibitory Activity.