Mass Production of Bovine Serum Albumin

Authors : Tha Zin Hlaing

Volume/Issue : Volume 4 - 2019, Issue 8 - August

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Bovine serum albumin ( BSA ) extracted from the whole blood of cows and oxen from slaughter house in Thanlyin Township, Yangon Division during the study period from July 2004 to November 2007. Its lyophilized powder was prepared and their yield, purity and potency were tested. Polyacrylamide gel electrophoresis (PAGE) demonstrated the comparable purity of 10 % locally prepared BSA solution and imported BSA. Overall results in Enzyme- Linked Immuno Sorbent Assay (ELISA) showed that optical density (OD) values, for IgG and IgM (anticardiolipin) in normal and patient sera, were observed to be lower when local BSA was used compared to purchase commercial BSA.

Keywords : Bovine Serum Albumin, Lyophilized Powder, Polyacrylamide Gel Electrophoresis, Enzyme- Linked Immuno Sorbent Assay.


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