Authors : In Sokra; Chey Socheata; Horn Meta; Rin Chanra; Horn Linan

Volume/Issue : Volume 9 - 2024, Issue 12 - December

Google Scholar : https://tinyurl.com/22eec826

Scribd : https://tinyurl.com/rcv9k8wj

DOI : https://doi.org/10.5281/zenodo.14471935

Abstract : Pathogenic fungi significantly damage crops in both vegetable and fruit cultivation. Trichoderma asperellum is a biocontrol fungus that suppresses these pathogens and promotes plant growth. However, its effectiveness in controlling such pathogens is limited. This study aimed to enhance the biocontrol efficacy of T. asperellum against crop-damaging fungi through metabolic adaptation. Serial transfer experiments were conducted in potato dextrose broth (PD broth) containing 3% sugar, followed by plating with anthracnose to compare the inhibitory effects of the wild-type strain and its evolved variant. The evolved strain, T. asperellum TIS-11T, demonstrated significantly improved growth and sugar utilization, reaching a biomass of 0.5 g/mL within 48 hours. Additionally, the evolved strain exhibited complete inhibition (100%) of anthracnose on PDA culture plates within 5 days. One-way ANOVA revealed a significant difference (p<0.01) increase in growth inhibition by the T. asperellum TIS-11T strain compared to the wild- type. In conclusion, T. asperellum TIS-11T is a highly effective biocontrol agent against anthracnose and has strong potential for industrial applications in pathogen management.

Keywords : Metabolic Adaptation, Trichoderma Asperellum, Growth Rate, Biocontrol, Anthracnose.

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