The methanolysis transesterification of Beniseed oil to biodiesel using acid activated waste cow bone was carried out. Solvent extraction was used to extract oil from the seeds using n-hexane and characterized to determine its physiochemical properties. The catalyst was synthesized from waste cow bone by activating it with concentrated phosphoric acid (H3PO4) at a ratio of 1:4 (w/v) on weight basis. It was dried in a Memmert oven at 100 oC for 2 hours and characterized to determine its catalytic properties. The effects of process parameters on biodiesel yield were examined. The biodiesel was characterized to determine its physiochemical properties. The result showed that the seed yielded 54 % oil. The low acid value and free fatty acid value of the oil enable it to undergo direct transesterification without treatment with the acid activated waste bone. The transesterification of the oil gave a maximum yield between 75 – 80% and the process parameters significantly affected the biodiesel yield. The properties of the biodiesel were within acceptable standard

Keywords : Acid Activated Waste Bone, Biodiesel, Soxhlet Extraction, Beniseed Oil, Transesterification Reaction.