A rapid, economic, sensitive, simple and
accurate first order derivative UV spectrophotometric
method has been developed for estimation of Etofylline
(ETO), Theophylline (THE) and Montelukast (MON) in
bulk and tablet dosage form. The quantitation of selected
wavelengths were 243 nm for ETO (zero cross for MON
and THE), 286 nm for THE (zero cross for MON but
shows absorbance in ETO) and 382 nm for MON (zero
cross for ETO and THE). It obeyed beer’s law in the
concentration range of 46.2-231 μg/ ml, 13.8-69 μg/ ml and
2-10 μg/ ml for ETO, THE and MON respectively. The
method was validated as per ICH guidelines.
Keywords : Etofylline, Theophylline, Montelukast, First order derivative method and Tablet dosage form.