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Microplastic Associated Microorganisms: Isolation, Identification and Assessment of Biofilm-Based Degradation Potential


Authors : Lakshmipriya V. P.; Mithra K.

Volume/Issue : Volume 11 - 2026, Issue 5 - May


Google Scholar : https://tinyurl.com/yc44svsu

Scribd : https://tinyurl.com/67pvc7r9

DOI : https://doi.org/10.38124/ijisrt/26May1957

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Microplastics have become major environmental contaminants because of their persistence and extensive distribution in both terrestrial and aquatic ecosystems. The present study aimed to isolate and identify microorganisms associated with microplastics and to evaluate their potential for biofilm-mediated plastic degradation. Soil and water samples were collected from plastic-polluted sites, and microplastics were extracted using density separation followed by hydrogen peroxide digestion. Fourier Transform Infrared (FTIR) spectroscopy confirmed the presence of polyolefin-based microplastics in the collected samples. Associated bacterial and fungal isolates were characterized through morphological and biochemical analyses. The biofilm-forming ability of the isolates was assessed using Congo Red Agar and tube assay methods. Furthermore, selected microbial isolates demonstrated noticeable plastic degradation potential based on weight loss measurements of treated plastic samples. The findings of this study emphasize the significant role of indigenous microorganisms in the sustainable bioremediation of microplastic pollution.

Keywords : Microplastics; Bioremediation; Biofilm Formation; Plastic Degradation; FTIR Analysis

References :

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Microplastics have become major environmental contaminants because of their persistence and extensive distribution in both terrestrial and aquatic ecosystems. The present study aimed to isolate and identify microorganisms associated with microplastics and to evaluate their potential for biofilm-mediated plastic degradation. Soil and water samples were collected from plastic-polluted sites, and microplastics were extracted using density separation followed by hydrogen peroxide digestion. Fourier Transform Infrared (FTIR) spectroscopy confirmed the presence of polyolefin-based microplastics in the collected samples. Associated bacterial and fungal isolates were characterized through morphological and biochemical analyses. The biofilm-forming ability of the isolates was assessed using Congo Red Agar and tube assay methods. Furthermore, selected microbial isolates demonstrated noticeable plastic degradation potential based on weight loss measurements of treated plastic samples. The findings of this study emphasize the significant role of indigenous microorganisms in the sustainable bioremediation of microplastic pollution.

Keywords : Microplastics; Bioremediation; Biofilm Formation; Plastic Degradation; FTIR Analysis

Paper Submission Last Date
30 - June - 2026

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