Authors :
Lakshmipriya V. P.; Mithra K.
Volume/Issue :
Volume 11 - 2026, Issue 5 - May
Google Scholar :
https://tinyurl.com/yc44svsu
Scribd :
https://tinyurl.com/67pvc7r9
DOI :
https://doi.org/10.38124/ijisrt/26May1957
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
Microplastics have become major environmental contaminants because of their persistence and extensive
distribution in both terrestrial and aquatic ecosystems. The present study aimed to isolate and identify microorganisms
associated with microplastics and to evaluate their potential for biofilm-mediated plastic degradation. Soil and water
samples were collected from plastic-polluted sites, and microplastics were extracted using density separation followed by
hydrogen peroxide digestion. Fourier Transform Infrared (FTIR) spectroscopy confirmed the presence of polyolefin-based
microplastics in the collected samples. Associated bacterial and fungal isolates were characterized through morphological
and biochemical analyses. The biofilm-forming ability of the isolates was assessed using Congo Red Agar and tube assay
methods. Furthermore, selected microbial isolates demonstrated noticeable plastic degradation potential based on weight
loss measurements of treated plastic samples. The findings of this study emphasize the significant role of indigenous
microorganisms in the sustainable bioremediation of microplastic pollution.
Keywords :
Microplastics; Bioremediation; Biofilm Formation; Plastic Degradation; FTIR Analysis
References :
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- G. D. Christensen, W. A. Simpson, J. J. Younger, L. M. Baddour, F. F. Barrett, D. M. Melton, and E. H. Beachey, Adherence of coagulase-negative staphylococci to plastic tissue culture plates: A quantitative model for the adherence of staphylococci to medical devices, Journal of Clinical Microbiology, vol. 22, no. 6, pp. 996–1006, 1985.
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- E. R. Zettler, T. J. Mincer, and L. A. Amaral-Zettler, Life in the ‘plastisphere’: Microbial communities on plastic marine debris, Environmental Science & Technology, vol. 47, no. 13, pp. 7137–7146, 2013.
Microplastics have become major environmental contaminants because of their persistence and extensive
distribution in both terrestrial and aquatic ecosystems. The present study aimed to isolate and identify microorganisms
associated with microplastics and to evaluate their potential for biofilm-mediated plastic degradation. Soil and water
samples were collected from plastic-polluted sites, and microplastics were extracted using density separation followed by
hydrogen peroxide digestion. Fourier Transform Infrared (FTIR) spectroscopy confirmed the presence of polyolefin-based
microplastics in the collected samples. Associated bacterial and fungal isolates were characterized through morphological
and biochemical analyses. The biofilm-forming ability of the isolates was assessed using Congo Red Agar and tube assay
methods. Furthermore, selected microbial isolates demonstrated noticeable plastic degradation potential based on weight
loss measurements of treated plastic samples. The findings of this study emphasize the significant role of indigenous
microorganisms in the sustainable bioremediation of microplastic pollution.
Keywords :
Microplastics; Bioremediation; Biofilm Formation; Plastic Degradation; FTIR Analysis