Authors : Subramaniam Manju

Volume/Issue : Volume 9 - 2024, Issue 11 - November

Google Scholar : https://tinyurl.com/37yx8che

Scribd : https://tinyurl.com/bs6t3nn3

DOI : https://doi.org/10.38124/ijisrt/IJISRT24NOV477

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Aim: This study investigates the antimicrobial potential of phytochemicals from Psoralea corylifolia against methicillin-resistant Staphylococcus aureus (MRSA) and elucidates the underlying mechanisms through molecular docking studies.  Methods: Mature seeds of Psoralea corylifolia were extracted using ethanol and chloroform via Soxhlet extraction. Qualitative analysis was performed to identify phytochemicals. Antibacterial activity was assessed using the agar well diffusion method against MRSA isolates, comparing results to gentamycin. GC-MS analysis characterized secondary metabolites, while molecular docking simulations with AutoDock Vina examined the binding affinity of (+)-Bakuchiol to the S. aureus receptor 1TSJ.  Results: Ethanol extraction proved superior, yielding a diverse range of phytochemicals, including flavonoids and alkaloids, while chloroform extraction was less effective. The ethanol extract exhibited significant antibacterial activity, with maximum zones of inhibition observed against S.aureus isolates. GC-MS identified key compounds, including (+)-Bakuchiol, which demonstrated a binding affinity of -5.8 kcal/mol in molecular docking studies. Interaction analysis highlighted critical amino acid residues involved in binding.  Conclusion: The findings confirm the efficacy of ethanol- extracted phytochemicals from Psoralea corylifolia, particularly (+)-Bakuchiol, as potential antibacterial agents against MRSA. These results support the need for further research into plant-derived compounds to combat antibiotic-resistant bacterial infections and offer insights into their mechanisms of action through molecular docking studies.

Keywords : Psoralea Corylifolia, MRSA, Ficusin, (+)- Bakuchiol, Isopsoralen, Molecular Docking.

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