Authors :
Subramaniam Manju
Volume/Issue :
Volume 9 - 2024, Issue 11 - November
Google Scholar :
https://tinyurl.com/37yx8che
Scribd :
https://tinyurl.com/bs6t3nn3
DOI :
https://doi.org/10.38124/ijisrt/IJISRT24NOV477
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
Aim:
This study investigates the antimicrobial potential
of phytochemicals from Psoralea corylifolia against
methicillin-resistant Staphylococcus aureus (MRSA) and
elucidates the underlying mechanisms through
molecular docking studies.
Methods:
Mature seeds of Psoralea corylifolia were extracted
using ethanol and chloroform via Soxhlet extraction.
Qualitative analysis was performed to identify
phytochemicals. Antibacterial activity was assessed using
the agar well diffusion method against MRSA isolates,
comparing results to gentamycin. GC-MS analysis
characterized secondary metabolites, while molecular
docking simulations with AutoDock Vina examined the
binding affinity of (+)-Bakuchiol to the S. aureus
receptor 1TSJ.
Results:
Ethanol extraction proved superior, yielding a
diverse range of phytochemicals, including flavonoids
and alkaloids, while chloroform extraction was less
effective. The ethanol extract exhibited significant
antibacterial activity, with maximum zones of inhibition
observed against S.aureus isolates. GC-MS identified key
compounds, including (+)-Bakuchiol, which
demonstrated a binding affinity of -5.8 kcal/mol in
molecular docking studies. Interaction analysis
highlighted critical amino acid residues involved in
binding.
Conclusion:
The findings confirm the efficacy of ethanol-
extracted phytochemicals from Psoralea corylifolia,
particularly (+)-Bakuchiol, as potential antibacterial
agents against MRSA. These results support the need for
further research into plant-derived compounds to
combat antibiotic-resistant bacterial infections and offer
insights into their mechanisms of action through
molecular docking studies.
Keywords :
Psoralea Corylifolia, MRSA, Ficusin, (+)- Bakuchiol, Isopsoralen, Molecular Docking.
References :
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Aim:
This study investigates the antimicrobial potential
of phytochemicals from Psoralea corylifolia against
methicillin-resistant Staphylococcus aureus (MRSA) and
elucidates the underlying mechanisms through
molecular docking studies.
Methods:
Mature seeds of Psoralea corylifolia were extracted
using ethanol and chloroform via Soxhlet extraction.
Qualitative analysis was performed to identify
phytochemicals. Antibacterial activity was assessed using
the agar well diffusion method against MRSA isolates,
comparing results to gentamycin. GC-MS analysis
characterized secondary metabolites, while molecular
docking simulations with AutoDock Vina examined the
binding affinity of (+)-Bakuchiol to the S. aureus
receptor 1TSJ.
Results:
Ethanol extraction proved superior, yielding a
diverse range of phytochemicals, including flavonoids
and alkaloids, while chloroform extraction was less
effective. The ethanol extract exhibited significant
antibacterial activity, with maximum zones of inhibition
observed against S.aureus isolates. GC-MS identified key
compounds, including (+)-Bakuchiol, which
demonstrated a binding affinity of -5.8 kcal/mol in
molecular docking studies. Interaction analysis
highlighted critical amino acid residues involved in
binding.
Conclusion:
The findings confirm the efficacy of ethanol-
extracted phytochemicals from Psoralea corylifolia,
particularly (+)-Bakuchiol, as potential antibacterial
agents against MRSA. These results support the need for
further research into plant-derived compounds to
combat antibiotic-resistant bacterial infections and offer
insights into their mechanisms of action through
molecular docking studies.
Keywords :
Psoralea Corylifolia, MRSA, Ficusin, (+)- Bakuchiol, Isopsoralen, Molecular Docking.