Authors : Ogudu, Emmanuel Ogbonnia; Udeani, Theophilus Kachi; Otuchukwu Nneka Alu; Chukwu-Oko Felicia Ogudu; Aniako Leonard Ubanagu

Volume/Issue : Volume 11 - 2026, Issue 3 - March

Google Scholar : https://tinyurl.com/3y7bdbcc

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DOI : https://doi.org/10.38124/ijisrt/26mar1698

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Abstract : Dengue virus infection is a global threat to public health due to its widespread morbidity and mortality. The incidence of dengue has grown dramatically around the world in recent decades. The aim of this study is to detect and characterize dengue fever virus infection among patients with febrile illness attending healthcare facilities in Ebonyi State, Nigeria. Those reporting with febrile conditions were recruited for the study excluding patients under two years of age. The study was carried out at the major primary health care centres and hospitals within Ebonyi State and the phlebotomy unit of each of the hospitals was used as the sample collection point. The study adopted a cross-sectional survey and a wellstructured questionnaire was used to obtain information from the patients. ELISA technique was used to presumptively detect the presence of dengue virus in the patient’s serum sample and then subsequently analysed molecularly using nested PCR technique to identify the different serotypes of the dengue virus. Other major febrile causing agents were also assessed to determine the level of co-infection with dengue virus. The other febrile agents assessed in this study are, Plasmodium falciparum, hepatitis C virus (HCV), hepatitis B virus (HBV) and enteric fever.

Keywords : Immunoglobulin, DENV, ELISA, IgM, IgG, HCV, HBV, and Plasmodium falciparum

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