Authors :
Muhammed Jamiu A; M. S. Abdulsalami; N.E. L. Egbe
Volume/Issue :
Volume 6 - 2021, Issue 11 - November
Google Scholar :
http://bitly.ws/gu88
Scribd :
https://bit.ly/3sHTUJO
Abstract :
Lacto ferrin is an iron chelating glycoprotein
of the transferrin family present in several biological
fluids. The study did a molecular identification,
quantitative analysis of LF from Camel, Goat and Sheep
milk andalso investigated the effect of the purified LF on
the growth inhibition of Staphylococcus aureus and
Escherichiacoli. Lactoferrin, was isolated from camel,
goat and sheep milk. Both isolation and subsequent
purification of the Lactoferrin from these sources using
CM Sephadex C-50, showed that camel milk contained
appreciable quantities of LF (2.60mg/ml).While Goat
milk and Sheep milk contained less quantities of LF as
compared to Camel milk (2.10mg/ml and 1.70mg/ml)
respectively. The result of bactericidal activity
demonstrate that Camel LF is bacteriocidal towards
S.aureus and E.coli. Minimal Inhibitory Concentrations
were seen even at higher concentrations and the least
concentration was at 0.60mg/ml of camel LF against
E.coli and 0.2mg/ml of camel LF against S.aureus. The
findings of this study Suggest that lactoferrin has
inhibitory potential against S. aureus and E. coli.
Lacto ferrin is an iron chelating glycoprotein
of the transferrin family present in several biological
fluids. The study did a molecular identification,
quantitative analysis of LF from Camel, Goat and Sheep
milk andalso investigated the effect of the purified LF on
the growth inhibition of Staphylococcus aureus and
Escherichiacoli. Lactoferrin, was isolated from camel,
goat and sheep milk. Both isolation and subsequent
purification of the Lactoferrin from these sources using
CM Sephadex C-50, showed that camel milk contained
appreciable quantities of LF (2.60mg/ml).While Goat
milk and Sheep milk contained less quantities of LF as
compared to Camel milk (2.10mg/ml and 1.70mg/ml)
respectively. The result of bactericidal activity
demonstrate that Camel LF is bacteriocidal towards
S.aureus and E.coli. Minimal Inhibitory Concentrations
were seen even at higher concentrations and the least
concentration was at 0.60mg/ml of camel LF against
E.coli and 0.2mg/ml of camel LF against S.aureus. The
findings of this study Suggest that lactoferrin has
inhibitory potential against S. aureus and E. coli.
