Molecular Typing of Staphylococcus Aureus Isolated from Various Environmental Sources

Authors : Ingilala Satheesh Kumar ; Nadakuditi Venkata Raju; Hanumohan R. Konatham

Volume/Issue : Volume 5 - 2020, Issue 6 - June

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DOI : 10.38124/IJISRT20JUN851

RAPD-PCR was employed for the characterization of S. aureus isolates from different sources such as soil, water, milk, meat and skin swab etc.,. Isolated pure cultures of S.aureus strains were subjected to genomic DNA isolation, purification, separation and quantification. Isolated DNA samples were distinguished by using 4 different random primers. Genome profile analysis obtained from the S.aureus demonstrated that it was possible to differentiate the S. aureus strains from different sources by RAPD technique. Results indicate possible relationships between host origin and genetic variation among S. aureus isolates from various sources. This RAPD method was thus useful for epidemiological studies of the S. aureus flora.

Keywords : Staphylococcus aureus, Genomic DNA, Random Amplified Polymorphic DNA (RAPD) and Polymerase Chain Reaction (PCR)


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