Authors : Shishir Srivastava; Meenakshee Pandey

Volume/Issue : Volume 10 - 2025, Issue 9 - September

Google Scholar : https://tinyurl.com/3596zb8w

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DOI : https://doi.org/10.38124/ijisrt/25sep810

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Abstract : This research delves into the fascinating interplay between nutrient availability, specifically nitrogen (N) and phosphorus (P), and the vitality of three crucial nitrogen-fixing cyanobacteria: Anabaena variabilis, Nostoc muscorum, and Aulosira fertilissima. These microscopic organisms play a pivotal role in rice paddy ecosystems, and understanding how they respond to varying nutrient levels is key to optimizing their beneficial functions. Our study involved cultivating these cyanobacterial strains under carefully controlled conditions, manipulating N and P concentrations to observe their impact on growth, chlorophyll-a production (a measure of photosynthetic health), heterocyst frequency (specialized cells for nitrogen fixation), nitrogenase activity (the enzyme responsible for nitrogen fixation), and phosphate uptake efficiency. The findings clearly indicate that a balanced nutrient supply—specifically, 25 mg/L of nitrogen and 2.5 mg/L of phosphorus— fosters optimal growth and metabolic efficiency in these cyanobacteria. Conversely, an overabundance of nitrogen was found to significantly hinder heterocyst formation and nitrogenase activity, essentially suppressing their ability to fix atmospheric nitrogen. These results underscore the critical importance of maintaining a harmonious nutrient environment, highlighting how phosphorus, in particular, acts as a vital catalyst for robust nitrogen fixation by cyanobacteria in the unique setting of rice paddy fields.

Keywords : Biomass, Chlorophyll-a Content, Diazotrophic Cyanobacteria, Heterocyst Frequency, Nitrogenase Activity, Phosphorus Uptake.

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