An attempt has been made to convert glucose into calcium gluconate with the help of Aspergillus niger through fermentation. The present study focused to optimise fermentation parameters like glucose concentration, dose of inoculums, age of inoculums, pH, agitation, aeration and temperature, for developing an efficient, cost-effective, eco-friendly protocol for the production of calcium gluconate, also examine the reproducibility of optimised protocol and scale-up study to decide its techno-commercial viability. The glucose fermentation protocol of various parameters optimized and subsequently an optimized protocol for the conversion of glucose into calcium gluconate was devised. It comprised (a) Optimal concentration of glucose 15%, (b) Optimal dose of inoculum 10%, (c) Optimal age of inoculum 24 hour, (d) Optimal pH 6 +0.2, (e) Optimal rate of agitation at 200 rpm, (f) Optimal rate of aeration 1.0 vvm, and (g) Optimal temperature 30+1 0C. By using this optimised protocol its scale up study showed that technocommercial viability was feasible and it can be commercialized without any problems with desire quality product as required by pharmaceutical, food, feed, poultry, and aquaculture industries due to its bioavailability. The currently preferred technology for the production of gluconic acid and its derivatives is with the aid of Aspergillus niger due to least pollution, less energy consumption, and higher productivity

Keywords : Calcium Gluconate, Gluconic Acid, Glucose, Fermentation, Aspergillus Niger.