Authors : Dr. Malasa H. Pai; Dr. Nagarathna D. V.

Volume/Issue : Volume 11 - 2026, Issue 3 - March

Google Scholar : https://tinyurl.com/2xaffbsn

Scribd : https://tinyurl.com/2287kucv

DOI : https://doi.org/10.38124/ijisrt/26mar2066

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Periodontitis is a complex inflammatory condition characterized by the gradual deterioration of the tissues that support the teeth, including the periodontal ligament and the alveolar bone. Timely diagnosis is crucial for preventing periodontitis. Diagnosing the condition involves various clinical and radiographic assessment methods that help classify the specific type of periodontitis affecting the patient. However, these methods have limitations; they do not provide realtime assessments of disease progression but instead indicate past tissue damage. Advancements in material science and nanotechnology have led to innovative approaches in periodontal diagnostics. One such development is the use of oral biosensors, which represent a significant leap in biomedical diagnostics and personalized medicine. Biosensors are compact, self-contained, analytical instruments designed to identify and quantify target substances. By enabling early detection and personalized treatment strategies, these biosensors hold great promise for improving patient outcomes and advancing oral healthcare.

Keywords : Artificial Intelligence (Ai) In Dentistry, Biosensors, Oral Nanobiosensors, Personalized Periodontics, Point-of-Care Test, Salivary Diagnostics, Salivary Biomarkers.

References :

1. Newman MG, Takei H, Klokkevold PR, Carranza FA. Newman and Carranza's Clinical Periodontology E-Book: Newman and Carranza's Clinical Periodontology E-Book. Elsevier Health Sci; 2018 May 29.
2. Bhalla N, Jolly P, Formisano N, Estrela P. Introduction to biosensors. Essays Biochem. 2016;1:10.
3. Clark LC Jr, Duggan CA. Implanted electroenzymatic glucose sensors. Diabetes Care. 1982 May 1;5(3):174-180.
4. Jurado-Sánchez B. Nanoscale biosensors based on self-propelled objects. Biosensors. 2018 Jun 25;8(3):59.
5. Ardila CM. Advancing healthcare through laboratory on a chip technology: Transforming microorganism identification and diagnostics. World J Clin Cases. 2025 Jan 26;13(3):97737.
6. Hossain N, Al Mahmud MZ, Hossain A, Rahman MK, Islam MS, Tasnim R, Mobarak MH. Advances of materials science in MEMS applications: A review. Results Eng. 2024 Jun 1;22:102115.
7. Shavanova K, Bakakina Y, Burkova I, Shtepliuk I, Viter R, Ubelis A, Beni V, Starodub N, Yakimova R, Khranovskyy V. Application of 2D non-graphene materials and 2D oxide nanostructures for biosensing technology. Sensors. 2016 Feb 6;16(2):223.
8. Taymour N, Hassan MG, AlGhamdi MA, Omara WS. From Detection to Treatment: Nanomaterial-Based Biosensors Transforming Prosthetic Dentistry and Oral Health Care: A Scoping Review. Prosthesis. 2025 May 14;7(3):51.
9. Kim CY, Shaban SM, Cho SY, Kim DH. Detection of periodontal disease marker with geometrical transformation of Ag nanoplates. Anal Chem. 2023 Jan 16;95(4):2356-65.
10. Bae G, Kim M, Lee A, Ji S, Jang M, Yim S, Song W, Lee SS, Yoon DH, An KS. Nanometric lamination of zinc oxide nanofilms with gold nanoparticles for self-perceived periodontal disease sensors. Compos Part B Eng. 2022 Feb 1;230:109490.
11. Zhang T, Xu Z, Liu X, Liu L, Jiang S, Zhang Z, Li Y, Pan S. Activated silver nanoparticle-based platform for specific capture of Porphyromonas gingivalis in human saliva. Sens Actuators B Chem. 2024 Mar 15;403:135171.
12. Yao M. Fluorescent labeling application of graphene oxide quantum dots in living human periodontal ligament stem cells. J Med Postgrad. 2020:587-91.
13. Griffith A, Chande C, Kulkarni S, Morel J, Cheng YH, Shimizu E, Cugini C, Basuray S, Kumar V. Point-of-care diagnostic devices for periodontitis–current trends and urgent need. Sens Diagn. 2024;3(7):1119-34.
14. Herr AE, Hatch AV, Giannobile WV, Throckmorton DJ, Tran HM, Brennan JS, Singh AK. Integrated microfluidic platform for oral diagnostics. Ann N Y Acad Sci. 2007 Mar;1098(1):362-74.
15. Hooshiar MH, Moghaddam MA, Kiarashi M, Al-Hijazi AY, Hussein AF, Alrikabi H, Salari S, Esmaelian S, Mesgari H, Yasamineh S. Recent advances in nanomaterial-based biosensor for periodontitis detection. J Biol Eng. 2024 Apr 18;18(1):28.
16. Umeizudike KA, Lähteenmäki H, Räisänen IT, Taylor JJ, Preshaw PM, Bissett SM, Tervahartiala T, Nwhator SO, Pärnänen P, Sorsa T. Ability of matrix metalloproteinase-8 biosensor, IFMA, and ELISA immunoassays to differentiate between periodontal health, gingivitis, and periodontitis. J Periodontal Res. 2022 Jun;57(3):558-67.
17. Taylor JJ, Jaedicke KM, van de Merwe RC, Bissett SM, Landsdowne N, Whall KM, Pickering K, Thornton V, Lawson V, Yatsuda H, Kogai T. A prototype antibody-based biosensor for measurement of salivary MMP-8 in periodontitis using surface acoustic wave technology. Sci Rep. 2019 Jul 30;9(1):11034.
18. Lowpradit P, Janmanee R, Tansriratanawong K. Performance Validation of Fabricated Nanomaterial-Based Biosensor for Matrix Metalloproteinase-8 Protein Detection. Eur J Dent. 2025 May 21.
19. Alassiri S, Parnanen P, Rathnayake N, Johannsen G, Heikkinen AM, Lazzara R, van der Schoor P, van der Schoor JG, Tervahartiala T, Gieselmann D, Sorsa T. The ability of quantitative, specific, and sensitive point-of-care/chair-side oral fluid immunotests for aMMP-8 to detect periodontal and peri-implant diseases. Dis Markers. 2018;2018(1):1306396.
20. Guida L, Bencivenga D, Annunziata M, Arcadio F, Borriello A, Della Ragione F, Formisano A, Piccirillo A, Zeni L, Cennamo N. An optical fiber-based point-of-care test for periodontal MMP-8 detection: A proof of concept. J Dent. 2023 Jul 1;134:104553.
21. Tortolini C, Gigli V, Angeloni A, Tasca F, Thanh NT, Antiochia R. A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis. Bioelectrochemistry. 2024 Apr 1;156:108590.
22. Zhu C, Zhao Y, Liu J. Sensitive detection of biomarker in gingival crevicular fluid based on enhanced electrochemiluminescence by nanochannel-confined Co3O4 nanocatalyst. Biosensors. 2025 Jan 19;15(1):63.
23. Cheng R, Wu Z, Li M, Shao M, Hu T. Interleukin-1β is a potential therapeutic target for periodontitis: a narrative review. Int J Oral Sci. 2020 Dec;12(1):2.
24. Kim JY, Kim KR, Kim HN. The potential impact of salivary IL-1 on the diagnosis of periodontal disease: a pilot study. Healthcare (Basel). 2021 Jun;9(6):729.
25. Cennamo N, Bencivenga D, Annunziata M, Arcadio F, Stampone E, Piccirillo A, Della Ragione F, Zeni L, Guida L, Borriello A. Plasmon resonance biosensor for interleukin-1β point-of-care determination: A tool for early periodontitis diagnosis. iScience. 2024 Jan 19;27(1).
26. Park R, Jeon S, Lee JW, Jeong J, Kwon YW, Kim SH, Jang J, Han DW, Hong SW. Mobile point-of-care device using molecularly imprinted polymer-based chemosensors targeting interleukin-1β biomarker. Biosensors. 2023 Dec 5;13(12):1013.
27. Singh P, Gupta ND, Bey A, Khan S. Salivary TNF-alpha: A potential marker of periodontal destruction. J Indian Soc Periodontol. 2014 May 1;18(3):306-10.
28. Varghese SS, Thomas H, Jayakumar ND, Sankari M, Lakshmanan R. Estimation of salivary tumor necrosis factor-alpha in chronic and aggressive periodontitis patients. Contemp Clin Dent. 2015 Sep 1;6(Suppl 1):S152-6.
29. Koley D. Electrochemical sensors for oral biofilm–biomaterials interface characterization: A review. Mol Oral Microbiol. 2022 Dec;37(6):292-8.
30. Tang Q, Zhang F, Xu W, Deng Z, Tang Z, Huang J. Advancements and Trends in Electrochemical Biosensors for Saliva-Based Diagnosis of Oral Diseases: A Bibliometric Analysis (2000–2023). Int Dent J. 2025 Aug 1;75(4):100840.
31. Jeon S, Kim SH, Heo G, Heo HJ, Chae SY, Kwon YW, Lee SK, Han DW, Kim HJ, Kim YH, Hong SW. A Wearable Electrochemical Biosensor for Salivary Detection of Periodontal Inflammation Biomarkers: Molecularly Imprinted Polymer Sensor with Deep Learning Integration. Adv Sci. 2025:e09658.
32. Wang K, Zheng X, Qi M, Zhang W, Du J, Han Q, Li C, Dong B, Wang L, Xu L. Flexible screen-printed electrochemical platform to detect hydrogen peroxide for the indication of periodontal disease. Sens Actuators B Chem. 2023 Sep 1;390:133955.
33. Kim J, Imani S, de Araujo WR, Warchall J, Valdés-Ramírez G, Paixão TR, Mercier PP, Wang J. Wearable salivary uric acid mouthguard biosensor with integrated wireless electronics. Biosens Bioelectron. 2015 Dec 15;74:1061-8.
34. Shi Z, Lu Y, Shen S, Xu Y, Shu C, Wu Y, Lv J, Li X, Yan Z, An Z, Dai C. Wearable battery-free theranostic dental patch for wireless intraoral sensing and drug delivery. NPJ Flex Electron. 2022 Jun 20;6(1):49.
35. Jundaeng J, Chamchong R, Nithikathkul C. Artificial intelligence-powered innovations in periodontal diagnosis: a new era in dental healthcare. Front Med Technol. 2025 Jan 10;6:1469852.
36. Wang J, Yu J, Wang T, Li C, Wei Y, Deng X, Chen X. Emerging intraoral biosensors. J Mater Chem B. 2020;8(16):3341-56.