Most of the microorganisms are known as beneficial to human and plants; one of them beneficial microorganism lactic acid bacteria (LAB) isolate cells and their cells free supernatants (CFS) have ability to inhibit the growth of pathogenic fungi and cells and their cells free supernatants (CFS) were observed that protect to the pepper CiliKulai seeds germination which are used worldwide by consumers. In this study treatment of small volumes cells and CFS of LAB isolates; Lb. plantarum1-MSS, P. pentosaceus1-MSS, Lb. acidophilus ATCC314, Lb. plantarum ATCC8014and Lb. plantarum1were showed to improve the CiliKulai seeds percentage germination; either seeds were infected artificially with Fusarium species; Fusarium sp. fus 124-FC,FusariumoxysporumKAML01-CL, Fusarium sp. CID124-CS and F. Proliferatum-LR is olates. In vitro assay with cells and CFS of LAB were assayed on pepper CiliKulai seeds germination in 160 eyes plastic tray. The highest percentage germination of seeds were noticed more than 97.00% when the soil was treated with Lb. plantarum1-FCF cells, even the Ciliseeds were infected with pathogenic fungi Fusarium sp. CID124-CSand F. proliferatum -LR. The pathogenic Fusarium species in fected with soil significantly (P≤0.05) improved percentage germination of CiliKulai seeds from 80.0 to 97.0% predominantly for seeds treated with CFSof Lb. plantarum1-FCF (98.0%). The percentage germination of seed was infected with Fusarium species were reduced the germination of seeds between 56.00% and 66.00% compare to control (96.00%), when grown in soil seeds infected with cells of pathogenic fungi Fusarium sp. fus 124-FC and Fusarium sp. CID124-CSgrown in soil without LAB cells. Effect of F. OxysporumKAML01-CL was noticed that highly virulent to suppress the seeds germination. However, all LAB cells and CFS were observed that to enhance the percentage germination.
Keywords : Cells and supernatant of Lactic Acid Bacteria; Fusarium Species; Inhibitory Activity; CiliKulai germination.