Phytochemical Analysis, Antioxidant Activity, and Antimicrobial Evaluation of Kinnow Fruit Peel Extract


Authors : Nivedita S M; Dr. Neelakanth M Jeedi

Volume/Issue : Volume 10 - 2025, Issue 7 - July


Google Scholar : https://tinyurl.com/4b85vwba

DOI : https://doi.org/10.38124/ijisrt/25jul227

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Abstract : This study investigates the bioactive constituents, antioxidant efficacy, and antimicrobial activities of kinnow fruit peel extract. Qualitative phytochemical analysis confirmed the presence of flavonoids, phenolics, tannins, and alkaloids. The extract’s antioxidant capacity, assessed using DPPH assay, exhibited notable radical scavenging activity. Antimicrobial properties were tested against E. coli and S. aureus using agar well diffusion technique, showing significant zones of inhibition. These results indicate the potential of kinnow peel as a natural therapeutic agent and sustainable by-product for the development of nutraceuticals and natural antimicrobial products.

Keywords : Phytochemicals Analysis, Antioxidant Activity, Antimicrobial Evaluation, Kinnow Fruit Peel Extract, Citrus Bioactives, Natural Therapeutics.

References :

  1. Yaqoob M., Aggarwal P., Babbar N. (2022). Extraction and screening of kinnow (Citrus reticulata L.) peel phytochemicals. Biomass Conversion and Biorefinery.
  2. Baswal A. K., et al. (2021). Edible coatings maintain phytochemicals in 'Kinnow' mandarin. Australian Journal of Crop Science, 15(10), 1332–1338.
  3. Purewal S. S., et al. (2022). Valorization of Kinnow peel bioactives. Journal of Food Measurement & Characterization, 17(1), 787–799.
  4. Gnanasaraswathi M., et al. (2014). Antioxidant behavior of citrus peels. J Chem Pharmaceut Sci, 2:139–144.
  5. Nimbal S. K., et al. (2024). Isolation and Identification of Cellulose producing Bacteria from Rotten Fruits. YMER, 23(09), 545–552.
  6. Rafiq, S., Singh, B., & Gat, Y. (2019). Effect of different drying techniques on chemical composition, color and antioxidant properties of kinnow (Citrus reticulata) peel. Journal of Food Science and Technology, 56(5), 2458–2466.
  7. Pulparambil, A., Rasane, P., Singh, J., Kaur, S., Bakshi, M., Mahato, D. K., Kaur, J., Gunjal, M., & Bhadariya, V. (2024). Bioactive Compounds from Kinnow Processing Waste and their Associated Benefits: A Review. Recent Advances in Food, Nutrition & Agriculture, 15(2), 103–114.
  8. Safdar, M. N., Kausar, T., Jabbar, S., Mumtaz, A., Ahad, K., & Saddozai, A. A. (2017). Extraction and quantification of polyphenols from kinnow (Citrus reticulate L.) peel using ultrasound and maceration techniques. Journal of Food and Drug Analysis, 25(3), 488–500.
  9. Saini, M. K., Neena Capalash, Varghese, E., Kaur, C., & Singh, S. P. (2022). A Targeted Metabolomics Approach to Study Secondary Metabolites and Antioxidant Activity in “Kinnow Mandarin” during Advanced Fruit Maturity. Foods, 11(10), 1410–1410.
  10. Abd-Elaal H A and Halaweish F T (2010) Food preservative activity of phenolic compounds in orange peel extracts (Citrus sinensis L.). Lucrari stiintifice Seria Zootehnie 53: 233-40.
  11. Ahmad M M, Salim-ur-Rehman Z, Iqbal F M A and Sultan J I (2006) Genetic variability to essential oil composition in four citrus species. Pak J Bot 38(2): 319- 24.
  12. Alia M, Horcajo C, Bravo L and Goya L (2003) Effects of grape antioxidant dietry fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. Nutr Res 23: 1251-67.
  13. Amarowicz R, Troszynska A, Barylko-Pikielna N and Shahidi F (2004) Extracts of polyphenolics from legume seeds- Correlation between their total antioxidant activity, total phenolics content, tannins content and astringency. J Food Lipid 11: 278-86.
  14. Amic D, Davidovic-Amic D, Beslo D and Trinajstic N (2003) Structure related scavenging activity relationship of flavonoids. Croat Chem Acta 76: 55-61.
  15. Anagnostopoulou M A, Kefalas P, Papageorgiou V P, Assimopoulou A N and Boskou D (2006) Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem 94: 19-25.
  16. Araujo C R R, Silva T M, Lopes M, Villela P, Alcantara A F C and Dessimoni-Pinto N A V (2013) Total antioxidant capacity, total phenolic content and mineral elements in the fruit peel of Myrciaria Cauliflora. Braz J Food Technol 16(4): 301-09. Arts I C W, Vande-Putte B and Hollman P C H (2000) Catechin contents of foods commonly consumed in the Netherlands. J Agric Food Chem 48: 1752-57.
  17. Chou C F and Huang Y L (2003) Comparison of the chemical composition and physicochemical properties of different fibers prepared from the peel of Citrus sinensis L. J Agric Food Chem 51: 2615-18.
  18. Cvetnic Z and Vladimir-Knezevic S (2004) Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Acta Pharm 54: 243-50.
  19. D’Archivio M, Filesi C, Di B R, Gargiulo R, Giovannini C and Masella R (2007)
  20. Polyphenols, dietry sources and bioavailability. Ann lst super sanita 43(4): 348-61.
  21. Dahmoune F, Boulekbache L, Moussi K, Aoun O, Spingo G and Madani K (2013) Volarization of citrus lemon residues for the recovery of antioxidants: Evaluation and optimization of microwave and ultrasound application to solvent extraction. Ind crop prod 50: 77-87.
  22. Ghasemi K, Ghasemi Y and Ebrahimzadeh M A (2009) Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak J Pharm Sci 22(3): 277-81.
  23. Giulia D C, Nicola M, Angelo A and Francesco C (1999) Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci 65: 337-53.
  24. Gnanasaraswathi M, Lakshmiprabha S, Rajadurai J R P, Abhinayashree M, Fathima B M, Aarthi L V and Kamatchi S (2014) Potent anti-oxidant behavior of citrus fruit peels and their bacterial activity against multi drug resistant organism Pseudomonas aeruginosa. J Chem Pharmaceut Sci 2: 139-44.

This study investigates the bioactive constituents, antioxidant efficacy, and antimicrobial activities of kinnow fruit peel extract. Qualitative phytochemical analysis confirmed the presence of flavonoids, phenolics, tannins, and alkaloids. The extract’s antioxidant capacity, assessed using DPPH assay, exhibited notable radical scavenging activity. Antimicrobial properties were tested against E. coli and S. aureus using agar well diffusion technique, showing significant zones of inhibition. These results indicate the potential of kinnow peel as a natural therapeutic agent and sustainable by-product for the development of nutraceuticals and natural antimicrobial products.

Keywords : Phytochemicals Analysis, Antioxidant Activity, Antimicrobial Evaluation, Kinnow Fruit Peel Extract, Citrus Bioactives, Natural Therapeutics.

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Paper Submission Last Date
31 - December - 2025

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